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2.A.1.3.50
Multidrug resistance protein 3 (Multidrug-efflux transporter 3) or Brm3, of 512 aas and 14 TMSs. Resistance to puromycin, tosofloxacin, norfloxacin, acriflavin, ethidium, and tetraphenyl phosphonium, but not ofloxacin, nalidixic acid or carbonyl cyanide m-chlorophenylhydrazone (Ohki and Murata 1997). A spontaneous B. subtilis mutant isolated in the presence of a high concentration of puromycin acquired a multidrug-resistant phenotype due to high level expression of the bmr3 gene (Ohki and Tateno 2004), and selection for improved synthesis of menaquinone-7 also caused increased expression (Cui et al. 2020).

Accession Number:P96712
Protein Name:Multidrug resistance protein 3
Length:512
Molecular Weight:55689.00
Species:Bacillus subtilis (strain 168) [224308]
Number of TMSs:14
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate tetraphenylphosphonium, norfloxacin, puromycin, 3,6-diamino-10-methylacridinium chloride, ethidium bromide, drug

Cross database links:

Entrez Gene ID: 938345   
Pfam: PF07690   
KEGG: bsu:BSU03070   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0046677 P:response to antibiotic
GO:0055085 P:transmembrane transport

References (3)

[1] “bmr3, a third multidrug transporter gene of Bacillus subtilis.”  Ohki R.et.al.   9023234
[2] “The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.”  Kunst F.et.al.   9384377
[3] “From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later.”  Barbe V.et.al.   19383706

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence 
1:	MDTTTAKQAS TKFVVLGLLL GILMSAMDNT IVATAMGNIV ADLGSFDKFA WVTASYMVAV 
61:	MAGMPIYGKL SDMYGRKRFF LFGLIFFLIG SALCGIAQTM NQLIIFRAIQ GIGGGALLPI 
121:	AFTIIFDLFP PEKRGKMSGM FGAVFGLSSV LGPLLGAIIT DSISWHWVFY INVPIGALSL 
181:	FFIIRYYKES LEHRKQKIDW GGAITLVVSI VCLMFALELG GKTYDWNSIQ IIGLFIVFAV 
241:	FFIAFFIVER KAEEPIISFW MFKNRLFATA QILAFLYGGT FIILAVFIPI FVQAVYGSSA 
301:	TSAGFILTPM MIGSVIGSMI GGIFQTKASF RNLMLISVIA FFIGMLLLSN MTPDTARVWL 
361:	TVFMMISGFG VGFNFSLLPA ASMNDLEPRF RGTANSTNSF LRSFGMTLGV TIFGTVQTNV 
421:	FTNKLNDAFS GMKGSAGSGA AQNIGDPQEI FQAGTRSQIP DAILNRIIDA MSSSITYVFL 
481:	LALIPIVLAA VTILFMGKAR VKTTAEMTKK AN