2.A.1.3.84
Trichothecene efflux pump, Tri12, of 590 aas and 14 TMSs (Lee et al. 2002). It may function as a phospholipid flippase, and five flippases (FgDnfA, B, C1, C2 and D have been identified  (Yun et al. 2020). FgDnfA is critical for normal vegetative growth while the other flippases are dispensable. FgDnfA and FgDnfD are crucial for fungal pathogenesis, and a remarkable reduction in deoxynivalenol (DON) production was observed in DeltaFgDNFA and DeltaFgDNFD strains. Deletion of the FgDNFB gene increased DON production to about 30 fold. FgDnfA and FgDnfD play positive roles in the regulation of trichothecene (TRI) gene (TRI1, TRI4, TRI5, TRI6, TRI12, and TRI101) expression and toxisome reorganization, while FgDnfB acts as a negative regulator of DON synthesis. FgDnfB and FgDnfD have redundant functions in the regulation of phosphatidylcholine transport, and double deletion of FgDNFB and FgDnfD showed defects in fungal development, DON synthesis, and virulence. Thus, the distinct and specific functions of flippase family members in F. graminearum have been determined, and FgDnfA, FgDnfD, and FgDnfB have specific spatiotemporal roles during toxisome biogenesis (Yun et al. 2020). This protein is 76% identical to the protein with TC# 2.A.1.3.47, and they probably catalyze the same reaction(s).