2.A.1.50.4
Multidrug efflux transporter, MET, of 507 aas and 12 TMSs (Chahine et al. 2012).  Exposure to dietary methotrexate was associated with increased fluid secretion rate and increased flux of methotrexate, but not salicylate. Exposure to methotrexate in the diet resulted in increases in the expression of a multidrug efflux transporter gene (MET; CG30344) in the Malpighian tubules. There were also increases in expression of genes for either a Drosophila multidrug resistance-associated protein (dMRP; CG6214; TC# 3.A.1.208.39) or an organic anion transporting polypeptide (OATP; CG3380; TC# 2.A.60.1.27), depending on the concentration of methotrexate in the diet.  MET probably does not export methotrexate (Chahine et al. 2012).