TCDB is operated by the Saier Lab Bioinformatics Group

  2.A.111 The Na+/H+ Antiporter-E (NhaE) Family

The NhaH family is a constituent of the IT superfamily (Lolkema and Slotboom 2003; Prakash et al. 2003; Rabus et al. 1999). It is the st321/AitJ family of Lolkema and Slotboom (2003). It consists of proteins from Gram-negative bacteria (e.g., Leptospira, Azotobacter, Neisseria, Ralstonia, Chlorobium and Rhizobial species). The proteins are of about 480 aas with 12-14 putative TMSs.

An open reading frame from the genome of Neisseria meningitidis displaying similarity with the NhaE type of Na+/H+ antiporters was expressed in E. coli and characterized for sodium transport ability. The N. meningitidis antiporter (NmNhaE) was able to complement an E. coli strain devoid of Na+/H+ antiporters (KNabc) with respect to the ability to grow in the presence of high concentrations of NaCl or LiCl. Ion transport assays in everted vesicles prepared from the KNabc strain expressing NmNhaE from a plasmid confirmed its ability to translocate Na+ and Li+ (Sousa et al. 2013).

The generalized reaction catalyzed by NhaE is,

Na+ or Li+ (in) + H+ (out) ⇌ Na+ or Li+ (out) + H+ (in)

This family belongs to the: IT Superfamily.

References associated with 2.A.111 family:

Lolkema, J.S. and D.J. Slotboom. (2003). Classification of 29 families of secondary transport proteins into a single structural class using hydropathy profile analysis. J. Mol. Biol. 327: 901-909. 12662917
Melo, A.M., S.A. Lobo, F.L. Sousa, A.S. Fernandes, M.M. Pereira, G.O. Hreggvidsson, J.K. Kristjansson, L.M. Saraiva, and M. Teixeira. (2005). A nhaD Na+/H+ antiporter and a pcd homologues are among the Rhodothermus marinus complex I genes. Biochim. Biophys. Acta. 1709: 95-103. 16023073
Prakash, S., G. Cooper, S. Singhi, and M.H. Saier, Jr. (2003). The ion transporter superfamily. Biochim. Biophys. Acta. 1618: 79-92. 14643936
Rabus, R., D.L. Jack, D.J. Kelly, and M.H. Saier, Jr. (1999). TRAP transporters: an ancient family of extracytoplasmic solute-receptor-dependent secondary active transporters. Microbiology 145(Pt12): 3431-3445. 10627041
Sousa, P.M., M.A. Videira, T. Vorburger, S.T. Silva, J.W. Moir, J. Steuber, and A.M. Melo. (2013). The novel NhaE-type Na+/H (+) antiporter of the pathogenic bacterium Neisseria meningitidis. Arch. Microbiol. 195: 211-217. 23208205