TCDB is operated by the Saier Lab Bioinformatics Group
TCIDNameDomainKingdom/PhylumProtein(s)
2.A.117.1:  The (mainly) Proteobacterial Chlorhexidine Efflux (PCE) family
2.A.117.1.1









The Chlorhexidine drug-resistance exporter, AceI of 179 aas and 4 TMSs (Hassan et al. 2013) .  It is capable of exporting multiple drugs such as benzalkonium, dequalinium, proflavine, and acriflavine. (Hassan et al. 2015). The aceI gene is induced in A. baumannii by the short-chain diamines, cadaverine and putrescine. Membrane transport experiments conducted in whole cells of A. baumannii and Escherichia coli and also in proteoliposomes showed that AceI mediates the efflux of these short-chain diamines (polyamines) such as  when energized by an electrochemical gradient (Hassan et al. 2019), suggesting that they are the phsiological substrates of AceI. AceI can form dimers and is regulated at the transcriptional level by AceR (Bolla et al. 2020).

Bacteria
Proteobacteria
AceI of Acinetobacter baumannii
2.A.117.1.2









Chlorhexidine-responsive chlorhexadine exporter of 171 aas and 4 TMSs.

Bacteria
Proteobacteria
AceI of Pseudomonas aeruginosa
2.A.117.1.3









Chlorhexidine-responsive putative chlorhxidine exporter of 160 aas and 4 TMSs, AceI (Hassan et al. 2013). It confers resistance to both proflavine and acriflavine by an active efflux mechanism (Hassan et al. 2015). AceR is an activator of aceI gene expression when challenged with chlorhexidine (Liu et al. 2018). This system also exports polyamines (organic diamines) such as cadaverine and putrescine (and possibly spermidine with low affinity).  It is induced preferentially by cadaverine and putrescine, and to a much lesser extent by spermidine. An AceI-E15Q mutant is inactive (Hassan et al. 2019).

Bacteria
Proteobacteria
AceI of Salmonella typhi
2.A.117.1.4









Chlorhexidine-unresponsive putative drug exporter of 147 aas and 4 TMSs.

Bacteria
Proteobacteria
Drug exporter of Pseudomonas aeruginosa
2.A.117.1.5









Chlorhexidine-unresponsive putative drug exporter of 144 aas and 4 TMSs.

Bacteria
Proteobacteria
Drug exporter of Desulfovibrio vulgaris
2.A.117.1.6









Uncharacterized putative drug exporter of 134 aas and 4 TMSs.

Bacteria
Firmicutes
UP of Veillonella parvula
2.A.117.1.7









Uncharacterized putative drug exporter of 147 aas and 4 TMSs.

Bacteria
Proteobacteria
UP of Methylobacterium populi
2.A.117.1.8









Uncharacterized putative drug exporter of 179 aas and 4 TMSs.

Bacteria
Actinobacteria
UP of Micrococcus luteus
2.A.117.1.9









Uncharacterized putative drug exporter of 346 aas and 4 N-terminal TMSs with a long C-terminal hydrophilic domain.

Bacteria
Proteobacteria
UP of Pseudomonas fluorescens
2.A.117.1.10









Uncharacterized putative drug exporter of 148 aas and 4 TMSs.

Bacteria
Proteobacteria
Transmembrane poir domain-protein of Azospira oryzae (Dechlorosoma suillum)
2.A.117.1.11









Uncharacterized putative drug exporter of 140 aas and 4 TMSs.

Bacteria
Proteobacteria
UP of Shewanella hoihica
2.A.117.1.12









The Unknown Bacterial Transmembrane Pair (UBTP) family member of 146 aas and 4 TMSs.  Chlorhexidine-unresponsive (Hassan et al. 2013).

Bacteria
Proteobacteria
UBTP family member of Burkholderia cenocepacia
2.A.117.1.13









Member of the Proteobacterial Antimicrobial Compound Efflux (PACE) family.  This protein is of 140 aas with 4 TMSs.  It has been shown to be an active drug exporter, conferring resistance to both proflavine and acriflavine, mediated by an active efflux mechanism (Hassan et al. 2018).

Bacteria
Proteobacteria
UBTP2 family member of Vibrio parahaemolyticus
2.A.117.2.1









Uncharacterized putative drug exporter of 142 aas and 4 TMSs.

Bacteria
Proteobacteria
UP of Rhodobacter spheroides
2.A.117.2.2









Uncharacterized protein of 148 aas and 4 TMSs in a 2 + 2 TMS arrangement.

Bacteria
Proteobacteria
UP of Hyphomicrobium sp. (freshwater metagenome)
2.A.117.2.3









PACE efflux transporter protein of 153 aas and 4 TMSs in a 2 + 2 TMS arrangement.

Bacteria
PVC group
PACE exporter of Planctomycetes bacterium (activated sludge metagenome)
2.A.117.3.1









Uncharacterized protein of 88 aas and 2 TMSs. Its sequence correspondes to the N-terminus of full length homologues and may therefore be a truncated version of a 4 TMS proteins.

Bacteria
Proteobacteria
UP of Yangia sp. PrR003