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2.A.17.4.4
The renal brush-border electrogenic, proton-coupled, broad specificity, high affinity, peptide transporter, PepT2 (Rubio-Aliaga et al., 2000). It is competitively inhibited by L-4,4'-Biphenylalanyl-L-Proline (Bip-Pro) with ~10-20µM affinity. Inhibitor/substrates includes cefadroxil, Ala-4-nitroanilide and delta-aminolevulinic acid (Knutter et al., 2007).  It transports the fluorescent tracer-dipeptide beta-Ala-Lys-Nepsilon-7-amino-4-methyl-coumarin-3-acetic acid (Ala-Lys-AMCA). Whole-mount preparations from mouse, rat, and guinea pig stomach and small and large intestine were incubated with Ala-Lys-AMCA in the presence or absence of the uptake-inhibitors L-histidine, D-phenylalanyl-L-alanine (D-Phe-Ala), glycyl-L-sarcosine (Gly-Sar), glycyl-L-glutamine (Gly-Gln), benzylpenicillin, and cefadroxil. Fluorescence microscopy revealed that Ala-Lys-AMCA specifically accumulated in both ganglionic layers of the enteric nervous system (ENS) in all regions and species studied (Rühl et al. 2005). This could be inhibited by Gly-Sar, D-Phe-Ala, Gly-Gln, and cefadroxil, but not by free histidine and benzylpenicillin, indicating uptake via PEPT2. Accordingly, dipeptide uptake was completely abolished in PEPT2-deficient mice.

Accession Number:Q9ES07
Protein Name:Solute carrier family 15 member 2
Length:729
Molecular Weight:81632.00
Species:Mus musculus [10090]
Number of TMSs:11
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate peptides

Cross database links:

Pfam: PF00854   

Gene Ontology

GO:0005887 C:integral to plasma membrane
GO:0005624 C:membrane fraction
GO:0015334 F:high affinity oligopeptide transporter acti...
GO:0015293 F:symporter activity
GO:0015031 P:protein transport

References (1)

[1] “Cloning and characterization of the gene encoding the mouse peptide transporter PEPT2.”  Rubio-Aliaga I.et.al.   11027540

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MNPFQKNESK ETLFSPVSTE EMLPGPPSPP KKSTPKLFGS SYPLSIAFIV VNEFCERFSY 
61:	YGMKAVLTLY FLYFLHWNED TSTSVYHAFS SLCYFTPILG AAIADSWLGK FKTIIYLSLV 
121:	YVLGHVFKSL GAIPILGGKM LHTILSLVGL SLIALGTGGI KPCVAAFGGD QFEEEHAEAR 
181:	TRYFSVFYLS INAGSLISTF ITPMLRGDVK CFGEDCYALA FGIPGLLMVL ALVVFAMGSK 
241:	MYRKPPPEGN IVAQVTKCIW FAICNRFRNR SEDIPKRQHW LDWAAEKYPK HLIMDVKALT 
301:	RILFLYIPLP MFWALLDQQG SRWTLQANKM DGDLGFFVLQ PDQMQVLNPF LVLVFIPLFD 
361:	LVIYRLISKC GVNFSSLRKM AVGMILACLA FAVAALVEIK INGMIHPQPA SQEIFLQVLN 
421:	LADGEIEVTV QGNRNNPLLV ESISSFQNTT HYSKLRLETK SQDLHFHLKY NNLSVHNEYS 
481:	VEEKNCYQLV VHENGESLSS MLVKDTGIKP ANGMTAIRFI NTLHKDMNIS LDANAPLSVG 
541:	KDYGVSEYRT VQRGKYPAVH CETEDNVFSL NLGQLDFGTT YLFVITNITN RGLQAWKAED 
601:	IPANKLSIAW QLPQYVLVTA AEVMFSVTGL EFSYSQAPSS MKSVLQAAWL LTVAVGNIIV 
661:	LIVAQFSGLV QWAEFVLFSC LLLVVCLIFS VMGYYYVPLK SEGIHEATEK QIPHIQGNMI 
721:	NLETKNTRL