2.A.20.2.14
Phosphate uptake transporter of 869 aas and 10 TMSs, 6 N-terminal and 4 C-terminal. Toxoplasma expresses one Pi transporter harboring two PHO4 binding domains (N- and C-terminal) that typify the PiT Family. This transporter named TgPiT, localizes to the plasma membrane, the inward buds of the endosomal organelles termed VAC, and many cytoplasmic vesicles (Asady et al. 2020). It catalyzes Pi:Na+ = 1:2 iselectrogenically. Upon Pi limitation in the medium, TgPiT is more abundant at the plasma membrane. ΔTgPiT parasites accumulate 4-times more acidocalcisomes, storage organelles for phosphate, and exhibit many traits that differ from the wild type organims including poor virulence (Asady et al. 2020). Either PgPiT or PT2 is essential for growth (Cui et al. 2022).
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| Accession Number: | KYF39953.1 |
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| Protein Name: | KYF39953.1 putative phosphate transporter family protein [Toxoplasma gondii ARI] |
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| Length: | 870 |
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| Molecular Weight: | |
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| Species: | Toxoplasma gondii ARI [1074872] |
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| Number of TMSs: | 10 |
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| Substrate |
sodium(1+), phosphate(3-) |
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1: METVAELSAP SLAGAPGEYT WIVAVAGVTC FLTAFAIGAN DVANTFSSSV GSRAIPLWAA
61: IGMSAVLETV GATLLGGAVT DSIRSKIIDF EVFRETPSIL MTGMLCALVG AGLWLFLANH
121: LGLPVSTTHS IIGALLGFGL ASGNVRAVKW TQVAFIVGSW VAAPLAASAA GATIFVCMRR
181: LILRSRQPLR RAKRFLWIFI YLITLTFSVF LVFKNFFELN MSCDQMVAGG RVEHFEPCRI
241: SRWADAHSGT ALGIAVALSV ALTFVISCLV YRFAFYRVES YRRRQKRSSR TEPRDASEEG
301: TGPSHARPGG LLLTPRSCVA KEGERRPGAL EDALRLAGEE SARIASAESR LQKPPPLECM
361: DTCADSLQIN DGRAAAAKPD VGTAAQSPES RFAADPVGSP VPDRSVLAFS RSLAEDAQAA
421: FHASVQHCAS PRCSAASRGD SRYPLQRSTS DFSAFLSSPS SSVPPSSPSP SSTPSSPSAS
481: PRRPPSRPPV PRTCSPAPVS PSVPRAFAST ATHGHPPALS DTDADTPEGP DRRRQSAALA
541: GAKDAEASPL FADLAPHPER RDEVPAAKRE RDARRTLLLP PRVTGEAPEG FFAPTVSHDD
601: NGLVFVSQAD LADSLGSGVD EEEDAPRSWR GKLKAAWRSM PWFKDIHAEG STEDDLVARL
661: QTGAEVFDTE TELFFSACQV VSACMGCIAH SANDTANAIG PFAAILTVYQ SGSADSEIGS
721: PWYILLFGGL SMSLGLALLG YRVIKTVGVK LVKITPARGF SMELGAAWTV LIFSAIGIPL
781: STTHCAVGST VGVGLMEPKH PRRETGDGPV AEGEEPKKRA VQCPVINTAS VNWKLFGGVF
841: VSWIITIAFS ALVTAALFSF AAYSPRLVS