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2.A.3.8.1
L-type neutral amino acid transporter, LAT1 (Na+-independent) (prefers amino acids with branched or aromatic side chains: Phe, Ile, Leu, Val, Trp, His; catalyzes obligatory exchange with μM affinities on the outside and mM affinities on the inside [1000x difference]). Both LAT1 and LAT2 (2.A.3.8.6) catalyze uptake of S-nitroso-L-cysteine. These and other LAT family members are specifically inhibited by 2-aminobicyclo[2.2.1]heptane-2-carboxylic acid (Li and Whorton, 2005). Mediates tryptophan:kynurenine exchange (Kaper et al., 2007). Also transports thyroid hormones and their derivatives (Kinne et al., 2011; Krause and Hinz 2017; Krause and Hinz 2019).  The chicken orthologue transports thyrold hormones, especially T2, with low affinity (Nele Bourgeois et al. 2016).

Accession Number:Q63016
Protein Name:LAT1 aka SLC7A5 aka MPE16 aka TA1
Length:512
Molecular Weight:55903.00
Species:Rattus norvegicus (Rat) [10116]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cytoplasm1 / Multi-pass membrane protein2
Substrate Amino acids, Thyroid hormones, thyroid hormone derivatives

Cross database links:

RefSeq: NP_059049.1   
Entrez Gene ID: 50719   
Pfam: PF00324   
KEGG: rno:50719   

Gene Ontology

GO:0016324 C:apical plasma membrane
GO:0005829 C:cytosol
GO:0016021 C:integral to membrane
GO:0042605 F:peptide antigen binding
GO:0030154 P:cell differentiation
GO:0007399 P:nervous system development
GO:0015804 P:neutral amino acid transport
GO:0055085 P:transmembrane transport

References (6)

[1] “Expression cloning and characterization of a transporter for large neutral amino acids activated by the heavy chain of 4F2 antigen (CD98).”  Kanai Y.et.al.   9726963
[2] “TA1, a highly conserved oncofetal complementary DNA from rat hepatoma, encodes an integral membrane protein associated with liver development, carcinogenesis, and cell activation.”  Sang J.et.al.   7532544
[3] “Expression of a system L neutral amino acid transporter at the blood-brain barrier.”  Matsuo H.et.al.   11095508
[4] “Transcriptional regulation of the LAT-1/CD98 light chain.”  Padbury J.F.et.al.   15120633
[5] “Expression of LAT1 and LAT2 amino acid transporters in human and rat intestinal epithelial cells.”  Fraga S.et.al.   16027961
[6] “L-type amino acid transporter 1-mediated L-leucine transport at the inner blood-retinal barrier.”  Tomi M.et.al.   15980244

External Searches:

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MAVAGAKRRA VAAPATTAAE EERQAREKML EARRGDGADP EGEGVTLQRN ITLINGVAII 
61:	VGTIIGSGIF VTPTGVLKEA GSPGLSLVVW AVCGVFSIVG ALCYAELGTT ISKSGGDYAY 
121:	MLEVYGSLPA FLKLWIELLI IRPSSQYIVA LVFATYLLKP VFPTCPVPEE AAKLVACLCV 
181:	LLLTAVNCYS VKAATRVQDA FAAAKLLALA LIILLGFIQM GKDIGQGDAS NLHQKLSFEG 
241:	TNLDVGNIVL ALYSGLFAYG GWNYLNFVTE EMINPYRNLP LAIIISLPIV TLVYVLTNLA 
301:	YFTTLSTNQM LTSEAVAVDF GNYHLGVMSW IIPVFVGLSC FGSVNGSLFT SSRLFFVGSR 
361:	EGHLPSILSM IHPQLLTPVP SLVFTCVMTL MYAFSRDIFS IINFFSFFNW LCVALAIIGM 
421:	MWLRFKKPEL ERPIKVNLAL PVFFILACLF LIAVSFWKTP LECGIGFAII LSGLPVYFFG 
481:	VWWKNKPKWI LQVIFSVTVL CQKLMQVVPQ ET