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2.A.49.3.1
NO3-:H+ (or less efficiently, Cl-:H+) antiporter, ClC-A or CLCa (De Angeli et al., 2006; Bergsdorf et al., 2009). Responsible for nitrate uptake into vacuoles (Zifarelli and Pusch, 2009b).  It is up-regulated under salt stress conditions (Yang et al. 2018) and may play a role in nitrate and Cd2+ resistance (Liao et al. 2019). The cryoEM structure has been solved at 2.8 Å resolution. An almost identical sequence has Uniprot acc # P92941 and differs from the one included in TCDB between residues 380 and 430, possibly due to sequencing errors. The central nitrate is shifted by approximately 1.4 Å from chloride, which is likely caused by a weaker interaction between the anion and Pro160; the side chains of aromatic residues around the central binding site are rearranged to accommodate the larger nitrate (He et al. 2022). The regulatory mechanisms of AtCLCa by nucleotides and phospholipids have been proposed (Yang et al. 2018) and may play a role in nitrate and Cd2+ resistance (Liao et al. 2019). The cryoEM structure has been solved at 2.8 Å resolution. An almost identical sequence has Uniprot acc # P92941 and differs from the one included in TCDB between residues 380 and 430, possibly due to sequencing errors. The central nitrate is shifted by approximately 1.4 Å from chloride, which is likely caused by a weaker interaction between the anion and Pro160; the side chains of aromatic residues around the central binding site are rearranged to accommodate the larger nitrate (He et al. 2022). The regulatory mechanisms of AtCLCa by nucleotides and phospholipids have been proposed (Yang et al. 2023).

Accession Number:Q96325
Protein Name:Voltage-gated chloride channel aka CLC-A
Length:773
Molecular Weight:85444.00
Species:Arabidopsis thaliana (Mouse-ear cress)   [3702]
Number of TMSs:13
Location1 / Topology2 / Orientation3: Golgi apparatus membrane1 / Multi-pass membrane protein2
Substrate chloride, hydron, nitrate

Cross database links:

Pfam: PF00571    PF00654   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005247 F:voltage-gated chloride channel activity
GO:0006821 P:chloride transport
GO:0055085 P:transmembrane transport

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MDEDGNLQIS NSNYNGEEEG EDPENNTLNQ PLLKRHRTLS STPLALVGAK VSHIESLDYE 
61:	INENDLFKHD WRSRSKAQVF QYIFLKWTLA CLVGLFTGLI ATLINLAVEN IAGYKLLAVG 
121:	YYIAQDRFWT GLMVFTGANL GLTLVATVLV VYFAPTAAGP GIPEIKAYLN GIDTPNMFGF 
181:	TTMMVKIVGS IGAVAAGLDL GKEGPLVHIG SCIASLLGQG GPDNHRIKWR WLRYFNNDRD 
241:	RRDLITCGSA SGVCAAFRSP VGGVLFALEE VATWWRSALL WRTFFSTAVV VVVLRAFIEI 
301:	CNSGKCGLFG SGGLIMFDVS HVEVRYHAAD IIPVTLIGVF GGILGSLYNH LLHKVLRLYN 
361:	LINQKGKIHK VLLSLGVSLL HQLLFGLPFL ANEACDPTID EICPTNGRSG NFKQFNCPNG 
421:	YYNDLSTLLL TTNDDAVRKH FLFKHSYEFG MVSLWIFFGL YCILGLITFG IATPSGLFLP 
481:	IILMGSAYGR MLGTAMGSYT NIDQGLYAVL GAASLMAGSM RMTVSLCVIF LELTNNLLLL 
541:	PITMFVLLIA KTVGDSFNLS IYEIILHLKG LPFLEANPEP WMRNLTVGEL NDAKPPVVTL 
601:	NGVEKVANIV DVLRNTTHNA FPVLDGADQN TGTELHGLIL RAHLVKVLKK RWFLNEKRRT 
661:	EEWEVREKFT PVELAEREDN FDDVAITSSE MQLYVDLHPL TNTTPYTVVQ SMSVAKALVL 
721:	FRSVGLRHLL VVPKIQASGM SPVIGILTRQ DLRAYNILQA FPHLDKHKSG KAR