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2.A.51.1.3
Chromate-resistance efflux pump, ChrA of 416 aas and 11 or 12 TMSs. The chrA gene of Pseudomonas aeruginosa plasmid pUM505 encodes ChrA, which confers resistance to chromate by the energy-dependent efflux of chromate ions (Aguilera et al. 2004). Chromate-sensitive mutants were mostly point mutations affected amino acids clustered in the N-terminal half of ChrA, altering either cytoplasmic regions or transmembrane segments, and replaced residues moderately to highly conserved in ChrA homologs. PhoA and LacZ translational fusions were used to confirm the membrane topology at the N-terminal half of the ChrA protein (Aguilera et al. 2004).

Accession Number:P14285
Protein Name:ChrA
Length:416
Molecular Weight:44060.00
Species:Pseudomonas aeruginosa [287]
Number of TMSs:11
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate Chromate

Cross database links:

Pfam: PF02417   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015109 F:chromate transmembrane transporter activity
GO:0015703 P:chromate transport
GO:0046687 P:response to chromate

References (1)

[1] “Cloning, nucleotide sequence, and expression of the chromate resistance determinant of Pseudomonas aeruginosa plasmid pUM505.”  Cervantes C.et.al.   2152903

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSVANEESYR PSKATDATTE AVPPPMSYPQ LFARFLKFGL LAWGGPVAQI DMLRRELVDE 
61:	ERWISSKRFN KLLAVMQVLP GPEAHEICVH LGIRAKGRLG GVLAGLGFML PGFLLMFALS 
121:	WLYFQIEFVG TALGAAFLGV QAAVIALIVR AVHRIGEHIL LDRWLWVIAI VCALAAIGRV 
181:	DFWITLPAGG LVYALLVLNH RASALLVTLA AVALAAAVAL WAAPTAKLVE AVVQGQASVL 
241:	LIFASGLKAG LLTFGGAYTA IPFVRNDAVG RGWMTDGQFL DGLALSGVLP APLIIFATFV 
301:	GYVAGGPIGA VAMTVGVFLP AFAFSLIFYD RLEAVVENKR LHAFLDGVAA GVVGLIGATT 
361:	IDLAQVTAER VPSLTVGMSI FAAGLAFLYA WKNKLNVVVV ILAAGLAGWL VFPNQG