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2.A.6.2.1 Multidrug (acriflavin, doxorubicin, ethidium, rhodamine 6G, SDS, deoxycholate) resistance pump [required for normal chromosomal condensation and segregation as well as cell division] (Lau and Zgurskaya, 2005). Exports L-cysteine (Yamada et al., 2006).
Accession Number:	P24181
Protein Name:	AcrF aka EnvD B3266
Length:	1034
Molecular Weight:	111454.00
Species:	Escherichia coli [83333]
Number of TMSs:	12
Location¹ / Topology² / Orientation³:	Cell inner membrane¹ / Multi-pass membrane protein²
Substrate	rhodamine 6G, doxorubicin, 3,6-diamino-10-methylacridinium chloride, deoxycholate, ethidium

DIP:	DIP-9052N
RefSeq:	AP_003806.1 NP_417732.1
Entrez Gene ID:	947768
Pfam:	PF00873
BioCyc:	EcoCyc:ACRF-MONOMER ECOL168927:B3266-MONOMER
KEGG:	ecj:JW3234 eco:b3266
Gene Ontology GO:0016021 C:integral to membrane GO:0005886 C:plasma membrane GO:0005215 F:transporter activity GO:0007049 P:cell cycle GO:0051301 P:cell division GO:0006810 P:transport
References (5) [1] “Molecular analysis and nucleotide sequence of the envCD operon of Escherichia coli.” Klein J.R.et.al. 1720861 [2] “Molecular cloning and characterization of acrA and acrE genes of Escherichia coli.” Ma D.et.al. 8407802 [3] “The complete genome sequence of Escherichia coli K-12.” Blattner F.R.et.al. 9278503 [4] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.” Hayashi K.et.al. 16738553 [5] “Global topology analysis of the Escherichia coli inner membrane proteome.” Daley D.O.et.al. 15919996

UniProt (Universal Protein Resource)
CDD Search (Conserved Domain Database)

Predict TMSs (Predict number of transmembrane segments)

FASTA formatted sequence

1:	MANFFIRRPI FAWVLAIILM MAGALAILQL PVAQYPTIAP PAVSVSANYP GADAQTVQDT 
61:	VTQVIEQNMN GIDNLMYMSS TSDSAGSVTI TLTFQSGTDP DIAQVQVQNK LQLATPLLPQ 
121:	EVQQQGISVE KSSSSYLMVA GFVSDNPGTT QDDISDYVAS NVKDTLSRLN GVGDVQLFGA 
181:	QYAMRIWLDA DLLNKYKLTP VDVINQLKVQ NDQIAAGQLG GTPALPGQQL NASIIAQTRF 
241:	KNPEEFGKVT LRVNSDGSVV RLKDVARVEL GGENYNVIAR INGKPAAGLG IKLATGANAL 
301:	DTAKAIKAKL AELQPFFPQG MKVLYPYDTT PFVQLSIHEV VKTLFEAIML VFLVMYLFLQ 
361:	NMRATLIPTI AVPVVLLGTF AILAAFGYSI NTLTMFGMVL AIGLLVDDAI VVVENVERVM 
421:	MEDKLPPKEA TEKSMSQIQG ALVGIAMVLS AVFIPMAFFG GSTGAIYRQF SITIVSAMAL 
481:	SVLVALILTP ALCATLLKPV SAEHHENKGG FFGWFNTTFD HSVNHYTNSV GKILGSTGRY 
541:	LLIYALIVAG MVVLFLRLPS SFLPEEDQGV FLTMIQLPAG ATQERTQKVL DQVTDYYLKN 
601:	EKANVESVFT VNGFSFSGQA QNAGMAFVSL KPWEERNGDE NSAEAVIHRA KMELGKIRDG 
661:	FVIPFNMPAI VELGTATGFD FELIDQAGLG HDALTQARNQ LLGMAAQHPA SLVSVRPNGL 
721:	EDTAQFKLEV DQEKAQALGV SLSDINQTIS TALGGTYVND FIDRGRVKKL YVQADAKFRM 
781:	LPEDVDKLYV RSANGEMVPF SAFTTSHWVY GSPRLERYNG LPSMEIQGEA APGTSSGDAM 
841:	ALMENLASKL PAGIGYDWTG MSYQERLSGN QAPALVAISF VVVFLCLAAL YESWSIPVSV 
901:	MLVVPLGIVG VLLAATLFNQ KNDVYFMVGL LTTIGLSAKN AILIVEFAKD LMEKEGKGVV 
961:	EATLMAVRMR LRPILMTSLA FILGVLPLAI SNGAGSGAQN AVGIGVMGGM VSATLLAIFF 
1021:	VPVFFVVIRR CFKG

2.A.6.2.1
Multidrug (acriflavin, doxorubicin, ethidium, rhodamine 6G, SDS, deoxycholate) resistance pump [required for normal chromosomal condensation and segregation as well as cell division] (Lau and Zgurskaya, 2005). Exports L-cysteine (Yamada et al., 2006).

Cross database links:

Gene Ontology

References (5)

External Searches:

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2.A.6.2.1Multidrug (acriflavin, doxorubicin, ethidium, rhodamine 6G, SDS, deoxycholate) resistance pump [required for normal chromosomal condensation and segregation as well as cell division] (Lau and Zgurskaya, 2005). Exports L-cysteine (Yamada et al., 2006).

Cross database links:

Gene Ontology

References (5)

External Searches:

Analyze:

2.A.6.2.1
Multidrug (acriflavin, doxorubicin, ethidium, rhodamine 6G, SDS, deoxycholate) resistance pump [required for normal chromosomal condensation and segregation as well as cell division] (Lau and Zgurskaya, 2005). Exports L-cysteine (Yamada et al., 2006).