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2.A.67.1.4
High affinity glutathione uptake porter, Hgt1p or OPT2 of 877 aas and ~12 TMSs in a 6 + 6 TMS arrangement. TMS 9 plays a role in glutathione recognition (Thakur & Bachhawat et al., 2010; Thakur and Bachhawat 2015). OPT2 shows highest affinity for reduced glutathione but also transports oxidized glutathione and glutathione conjugates with lesser affinities (Zulkifli et al. 2016). Residues have been identified that are essential for H+-dependent glutathione uptake (Zulkifli and Bachhawat 2017).  OPT2 is upregulated to facilitate the uptake of oligopeptide and amino acid into yeast cells (Zhu et al. 2023).

Accession Number:Q06593
Protein Name:Opt2p aka YPR194c aka P9677.13
Length:877
Molecular Weight:101261.00
Species:Saccharomyces cerevisiae (Baker's yeast) [4932]
Number of TMSs:14
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate glutathione, glutathione disulfide, glutathione conjugate

Cross database links:

DIP: DIP-5482N DIP-5482N
RefSeq: NP_015520.1   
Entrez Gene ID: 856324   
Pfam: PF03169   
KEGG: sce:YPR194C   

Gene Ontology

GO:0005887 C:integral to plasma membrane
GO:0015198 F:oligopeptide transporter activity
GO:0005515 F:protein binding
GO:0006857 P:oligopeptide transport
GO:0015031 P:protein transport
GO:0055085 P:transmembrane transport
GO:0042144 P:vacuole fusion, non-autophagic

References (8)

[1] “The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.”  Bussey H.et.al.   9169875
[2] “Schizosaccharomyces pombe isp4 encodes a transporter representing a novel family of oligopeptide transporters.”  Lubkowitz M.A.et.al.   9643541
[3] “Hgt1p, a high affinity glutathione transporter from the yeast Saccharomyces cerevisiae.”  Bourbouloux A.et.al.   10788431
[4] “A global topology map of the Saccharomyces cerevisiae membrane proteome.”  Kim H.et.al.   16847258
[5] “The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.”  Bussey H.et.al.   9169875
[6] “Schizosaccharomyces pombe isp4 encodes a transporter representing a novel family of oligopeptide transporters.”  Lubkowitz M.A.et.al.   9643541
[7] “Hgt1p, a high affinity glutathione transporter from the yeast Saccharomyces cerevisiae.”  Bourbouloux A.et.al.   10788431
[8] “A global topology map of the Saccharomyces cerevisiae membrane proteome.”  Kim H.et.al.   16847258

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence 
1:	MSETVKDKVI IDEKVSTKGT VDYAEGAEYS ERLSNHSSDF SQWYTDEQIL HFMKKLGYEN 
61:	RTLYDIPEDV AYILKKMPEL TLEDSFKILK DSIIYFKDDE NIPHDQYEEW KRLVDLEDLD 
121:	SKEGIDEYDS FDIRAFASAI KFHSPYQEVR AVVDPEDDPT IPVETFRAYF LAIIWSVIGS 
181:	GFNEFFSHRV VSISLNTPII QMFLYICGKA WAKTIPCWTI TIRGRKYGIN IDKPWTQKEQ 
241:	MFSTLLYAIC QGAFYTHYNI LTQKLFYHSA FSFGYQFLLS LSVQFIGFGF AGILRKFVVY 
301:	PARALWPTVM PTIAINKALL GKEKHESGMS RYKFFFLTFF IMFIYNWFPT YIINILNTFN 
361:	WMTWIKPSNI NLANITGGVT GLGINPISSF DWNVISFNSP LVYPFWSYLT QYLGCILAAL 
421:	IVIAVYYSNY MSCQYLPIFT NSLYTNTGHS FKVTEVLDSD NKLDVKKYQS YSPPYYSAGN 
481:	LVSYGAFICA YPLMITWSFI VHSKLLFNAF KDWALNLWAM RKLKSWVTMF KSDYRALDDY 
541:	DDPHSNAMKN YKEVPDWWYF AILIGSLVVG IAVVEHYPTN TPVWGLFVCL GFNFVFLIPT 
601:	TILQATTGYS FGLNLLIEMV MGYALPGNPI AIMILKAFGY NIDGQADNYV SNLKIAHYCK 
661:	IPPMALFRGQ CVIVFIQIFV NLGVLNWQIS NIKDFCTPHQ NAKFTCPDAV TYYNASVVWG 
721:	AIGPKRIFNY IYPIFKWCWL IGACIGIFFG VWKRWGKFYP RYFDPMLFVG GMLNMSPPYN 
781:	LMYYTSGMIV SYISQYYMKR HHLNLWEKYN YVLSAGFSTG LVLSAIIIFF AVQYKDTAFN 
841:	WWGNTVPYAG ADGVGYPLKN ITDTANGYFG YAPGHYP