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2.A.76.1.5
The leucine exporter (LeuE; YeaS) (binds and probably transports L-methionine, L-histidine, L-α-amino-n-butyrate, norleucine, azaleucine, S(2-aminoethyl)-L-cysteine, 3,4-dehydro-DL-proline, L-alanine, and both D- and L-valine) (Kutukova et al., 2005; Hori et al., 2011; Radi et al. 2022).  Expression is under the control of the leucine-responsive protein, Lrp, and amino acids in the medium (Kutukova et al. 2005). Mutation of the leuE gene allows tolerance to high levels of histidine, presumably by allowing histidine efflux (Radi et al. 2022).

Accession Number:P76249
Protein Name:Hypothetical protein yeaS aka YeaS
Length:212
Molecular Weight:23201.00
Species:Escherichia coli [83333]
Number of TMSs:6
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate L-methionine, L-valine, leucine, L-histidine

Cross database links:

RefSeq: AP_002417.1    NP_416312.1   
Entrez Gene ID: 946157   
Pfam: PF01810   
BioCyc: EcoCyc:G6984-MONOMER    ECOL168927:B1798-MONOMER   
KEGG: ecj:JW1787    eco:b1798   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015190 F:L-leucine transmembrane transporter activity
GO:0015820 P:leucine transport

References (4)

[1] “A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map.”  Itoh T.et.al.   9097040
[2] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[3] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[4] “The yeaS (leuE) gene of Escherichia coli encodes an exporter of leucine, and the Lrp protein regulates its expression.”  Kutukova E.A.et.al.   16098526

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence 
1:	MFAEYGVLNY WTYLVGAIFI VLVPGPNTLF VLKNSVSSGM KGGYLAACGV FIGDAVLMFL 
61:	AWAGVATLIK TTPILFNIVR YLGAFYLLYL GSKILYATLK GKNSEAKSDE PQYGAIFKRA 
121:	LILSLTNPKA ILFYVSFFVQ FIDVNAPHTG ISFFILAATL ELVSFCYLSF LIISGAFVTQ 
181:	YIRTKKKLAK VGNSLIGLMF VGFAARLATL QS