TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


4.D.3.1.5
Cellulose synthase and transporter, BcsA, which functions with BcsB (periplasmic protein with an N-terminal TMS) and BcsC (an 18 β-stranded outer membrane porin). The x-ray structure of the BcsA-B complex has been determined at 3.5 Å resolution (Morgan et al. 2013).  Cellulose is synthesized and secreted by the membrane-integrated cellulose synthase.  Substrate- and product-bound structures of BcsA provided the basis for substrate recognition and demonstrated the stepwise elongation of cellulose. Structural snapshots showed that BcsA translocates cellulose via a ratcheting mechanism involving a 'finger helix' that contacts the polymer's terminal glucose. Cooperating with BcsA's gating loop, the finger helix moves 'up' and 'down' in response to substrate binding and polymer elongation, respectively, thereby pushing the elongated polymer into BcsA's transmembrane channel. This mechanism was validated by tethering BcsA's finger helix, which inhibits polymer translocation but not elongation (Morgan et al. 2016).

Accession Number:Q3J125
Protein Name:Putative cellulose synthase
Length:788
Molecular Weight:87963.00
Species:Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158) [272943]
Number of TMSs:8
Substrate Cellulose

Cross database links:

Structure:
4HG6   4P00   4P02   5EIY   5EJ1   5EJZ     

External Searches:

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
FASTA formatted sequence
1:	MTVRAKARSP LRVVPVLLFL LWVALLVPFG LLAAAPVAPS AQGLIALSAV VLVALLKPFA 
61:	DKMVPRFLLL SAASMLVMRY WFWRLFETLP PPALDASFLF ALLLFAVETF SISIFFLNGF 
121:	LSADPTDRPF PRPLQPEELP TVDILVPSYN EPADMLSVTL AAAKNMIYPA RLRTVVLCDD 
181:	GGTDQRCMSP DPELAQKAQE RRRELQQLCR ELGVVYSTRE RNEHAKAGNM SAALERLKGE 
241:	LVVVFDADHV PSRDFLARTV GYFVEDPDLF LVQTPHFFIN PDPIQRNLAL GDRCPPENEM 
301:	FYGKIHRGLD RWGGAFFCGS AAVLRRRALD EAGGFAGETI TEDAETALEI HSRGWKSLYI 
361:	DRAMIAGLQP ETFASFIQQR GRWATGMMQM LLLKNPLFRR GLGIAQRLCY LNSMSFWFFP 
421:	LVRMMFLVAP LIYLFFGIEI FVATFEEVLA YMPGYLAVSF LVQNALFARQ RWPLVSEVYE 
481:	VAQAPYLARA IVTTLLRPRS ARFAVTAKDE TLSENYISPI YRPLLFTFLL CLSGVLATLV 
541:	RWVAFPGDRS VLLVVGGWAV LNVLLVGFAL RAVAEKQQRR AAPRVQMEVP AEAQIPAFGN 
601:	RSLTATVLDA STSGVRLLVR LPGVGDPHPA LEAGGLIQFQ PKFPDAPQLE RMVRGRIRSA 
661:	RREGGTVMVG VIFEAGQPIA VRETVAYLIF GESAHWRTMR EATMRPIGLL HGMARILWMA 
721:	AASLPKTARD FMDEPARRRR RHEEPKEKQA HLLAFGTDFS TEPDWAGELL DPTAQVSARP 
781:	NTVAWGSN