8.A.82.5.1
Aequorin-1 of 196 aas and 0 TMSs. It is a Ca²⁺-dependent bioluminescence photoprotein that displays an emission peak at 470 nm (blue light). Trace amounts of calcium ion trigger the intramolecular oxidation of the chromophore, coelenterazine, into coelenteramide and CO₂ with the concomitant emission of light (). Characteristics of the aequorin-expressing yeast strains appear suitable for its use in expression-based methods directed at cloning Ca²⁺ transporters from mammalian mitochondria and for further examining the interrelationships between mitochondrial and cytoplasmic Ca²⁺ in yeast (Jung et al. 2004).