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9.C.8 The ABC Lignin Precursor Transporters (ALPT) Family

Lignin is a complex biopolymer derived primarily from the condensation of three monomeric precursors, the monolignols. The synthesis of monolignols occurs in the cytoplasm. To reach the cell wall where they are oxidized and polymerized, they must be transported across the cell membrane. Using isolated plasma and vacuolar membrane vesicles prepared from Arabidopsis, together with applying different transporter inhibitors in the assays, Miao and Liu (2010) examined the uptake of monolignols and their derivatives by these native membrane vesicles. They demonstrated that the transport of lignin precursors across plasmalemma and their sequestration into vacuoles are ATP-dependent primary-transport processes, involving ATP-binding cassette-like transporters. Moreover, both plasma and vacuolar membrane vesicles selectively transported different forms of lignin precursors. In the presence of ATP, the inverted plasma membrane vesicles preferentially took up monolignol aglycones, whereas the vacuolar vesicles were more specific for glucoconjugates, suggesting that the different ATP-binding cassette-like transporters recognize different chemical forms in conveying them to distinct sites, and that glucosylation of monolignols is necessary for their vacuolar storage but not required for direct transport to the cell wall in Arabidopsis (Liu et al., 2011). How do lignin precursors (monolignols) get from inside the cell out to the cell wall where they are polymerized? Modeling indicates that monolignols can passively diffuse through lipid bilayers (Perkins et al. 2022).

Lignin is an aromatic plant cell wall polymer that facilitates water transport through the vasculature of plants. Lignin has the ability to reduce bacterial growth (Grossman et al. 2022). Lignin damages the S. aureus cell membrane, causes increased cell clustering, and inhibits growth synergistically with tunicamycin, a teichoic acid synthesis inhibitor. Lignin restored the susceptibility of genetically resistant S. aureus isolates to penicillin and oxacillin, decreased the intracellular pH, impaired normal cell division, and rendered cells more resistant to detergent-induced lysis. Transcriptome sequencing (RNA-Seq) differential expression (DE) analysis of lignin-treated cultures revealed significant gene expression changes (P < 0.05 with a 5% false discovery rate related to the cell envelope, cell wall physiology, fatty acid metabolism, and stress resistance. Moreover, a pattern of concurrent up- and downregulation of genes within biochemical pathways involved in transmembrane transport and cell wall physiology was observed, which likely reflects an attempt to tolerate or compensate for lignin-induced damage (Grossman et al. 2022).

References associated with 9.C.8 family:

Grossman, A.B., W. Vermerris, and K.C. Rice. (2022). Dysregulation of Cell Envelope Homeostasis in Staphylococcus aureus Exposed to Solvated Lignin. Appl. Environ. Microbiol. 88: e0054822. 35852361
Liu, C.J., Y.C. Miao, and K.W. Zhang. (2011). Sequestration and transport of lignin monomeric precursors. Molecules 16: 710-727. 21245806
Miao, Y.C. and C.J. Liu. (2010). ATP-binding cassette-like transporters are involved in the transport of lignin precursors across plasma and vacuolar membranes. Proc. Natl. Acad. Sci. USA 107: 22728-22733. 21149736
Perkins, M.L., M. Schuetz, F. Unda, K.T. Chen, M.B. Bally, J.A. Kulkarni, Y. Yan, J. Pico, S.D. Castellarin, S.D. Mansfield, and A.L. Samuels. (2022). Monolignol export by diffusion down a polymerization-induced concentration gradient. Plant Cell 34: 2080-2095. 35167693