1.A.100 The Rhabdoviridae Putative Viroporin, U5 (RV-U5) Family 

Viruses belonging to the family Rhabdoviridae infect a variety of different hosts including insects, vertebrates and plants. There are over 200 rhabdoviruses isolated around the world.  The complete genome sequence and predicted transcription strategy of Wongabel virus (WONV), a  rhabdovirus isolated from biting midges (Culicoides austropalpalis) is available. The 13,196 nucleotide genome encodes five typical rhabdovirus genes N, P, M, G and L, plus three genes located between the P and M genes (U1, U2, U3) and two overlapping the N and G genes (U4, U5). The U5 gene product has characteristics typical of viroporins with structural similarities with the alpha-1 protein (putative viroporin) of viruses in the genus Ephemerovirus, and similarity with the avian-associated Flanders virus has been noted (Gubala et al. 2008). 

Viroporins are viral ion channel proteins usually with 50-120 amino acids.  They play important roles in regulating virus replication, including virus entry, assembly and release. They may also modulate the electrochemical balance in subcellular compartments of the host cells. Wang et al. 2011 summarized advances concerning  viroporins, including PBCV-1 KcV, influenza M2, HIV-1 Vpu, HCV p7, picornavirus 2B, and the coronavirus E and 3a viroporins with emphasis on their functions and mechanisms.

This family belongs to the Phospholemman/SIMP/Viroporin (PSV) Superfamily.



Gubala, A.J., D.F. Proll, R.T. Barnard, C.J. Cowled, S.G. Crameri, A.D. Hyatt, and D.B. Boyle. (2008). Genomic characterisation of Wongabel virus reveals novel genes within the Rhabdoviridae. Virology 376: 13-23.

Wang, K., S. Xie, and B. Sun. (2011). Viral proteins function as ion channels. Biochim. Biophys. Acta. 1808: 510-515.


TC#NameOrganismal TypeExample

The Wongabel virus U5 protein (putative viroporin) of 127 aas and 1 or 2 TMSs.

U5 of Wongabel virus


Putative viroporin of 118 aas and 1 or 2TMSs

Putative Viroporin of Joinjakaka virus


Putative viroporin of 90 aas and 1 TMS

Putative viroporin of Kotonkan virus


Uncharacterized protein of 105 aas and 1 TMS.

UP of Parry Creek virus


alpha1 (α1) protein of 90 aas and 1 TMS

alpha1 protein of Koolpinyah virus


TC#NameOrganismal TypeExample

TC#NameOrganismal TypeExample