1.A.92 The Reovirus Viroporin VP10 (RVP10) Family Scylla serrata reovirus (SsRV) is a pathogen that can severely damage mud crab (Scylla serrata) aquaculture. Protein p35 with 2 TMSs, also called VP10, is encoded by the S10 gene. SsRV p35 can induce membrane permeability changes when expressed in Escherichia coli. Expressed in bacterial cells, it exists as monomers under reducing conditions but forms homodimers and homotrimers under non-reducing conditions. p35 therefore has the properties of a viroporin. It may resemble NS3-like proteins (TC#9.B.66).
This family belongs to the Viroporin-1.
References:
Yuan, Y., D. Fan, S. Zhu, J. Yang, and J. Chen. (2017). Identification and characterization of host cell proteins interacting with Scylla serrata reovirus non-structural protein p35. Virus Genes 53: 317-322.
Zhang, Z., Y. Yuan, D. Fan, J. Yang, Z. Mao, Y. Yan, and J. Chen. (2015). Scylla serrata reovirus p35 protein expressed in Escherichia coli cells alters membrane permeability. Virus Genes 51: 69-76.
Examples:
TC#	Name	Organismal Type	Example
1.A.92.1.1	Scylla serrata reovirus of 323 aas and 2 N-terminal TMSs, p35 or VP10, encoded by the S10 gene. This proposed viroporin seems to transport ions in a channel-type mechanism (Zhang et al. 2015). This putative viroporin interacts with host proteins (Yuan et al. 2017).		*p35 of Scylla serrata* reovirus**

1.A.92.1.2	Uncharaterized protein of 323 aas and 2 or 3 N-terminal TMSs.		UP of Eriocheir sinensis reovirus

1.A.92.1.3	VP10 of 340 aas and 2 or 3 N-terminal TMSs. This protein is 73% identical to 1.A.92.1.2.		*VP10 of Eriocheir sinensis* reovirus**