Gasdermin bGSDM of 259 aas and 1 possible TMS at residue 85. It is the precursor of a pore-forming protein involved in defense against bacteriophages. Expression of gasdermin bGSDM and the neighboring protease gene (Ga0098714_109514) is toxic in E.coli on solid medium (Johnson et al. 2022). Cleavage of this precursor by its dedicated protease releases the active moiety (gasdermin bGSDM, N-terminus), which inserts into membranes, forming pores and triggering cell death.

Gasdermin bGSDM of Bradyrhizobium tropiciagri 

Uncharacterized protein of 350 aas and 1 putative TMS.

UP of Streptomyces sp. DSM 40750

Uncharacterized protein of 233 aas and one possible TMS at about residue 80.

UP of Mangrovivirga sp. M17

Uncharacterized protein of 286 aas and possibly 1 or 2 TMSs.

UP of Methylobacteriaceae

Uncharacterized protein of 233 aas and possibly 3 TMSs, 2 between residues 80 and 130, and one C-terminal.

UP of Methanomicrobiales archaeon

Uncharacterized protein of 249 aas and possibly as many as 3 mderately hydrophobic TMSs, two between residues 150 and 190, and one more at the C-terminus.

UP of Candidatus Coatesbacteria bacterium

Uncharacterized protein of 258 aas and possibly 3 TMSs, two near the N-terminus (residues 10 - 50).  This protein shows sequence similarity with members of TC subclasses 1, 2, 4, and 5 in family 1.C.137.

UP of Pinibacter aurantiacus

Uncharacterized protein of 288 aas and possibly two TMSs, one N-terminal and one at about residue 120.

UP of Acidobacteriota bacterium (soil metagenome)

Uncharacterized protein of 267 aas and 1 possible TMS near the C-terminus of the protein.

UP of Ruegeria atlantica

Uncharacterized protein of 272 aas and 1 or 2 moderately hydrophobic TMSs near the C-terminus of the protein.

UP of Thiococcus pfennigii

Uncharacterized protein of 271 aas and possible 3 TMSs, one at about residue 90, and two between residues 190 and 225.

UP of Lysobacter enzymogenes

Uncharacterized protein of 267 aas and at least 1 possible TMS between residues 80 and 100, and possibly several less hydrophobic peaks towards the C-terminus of the protein.

UP of Spirosoma sp. KCTC 72228

Uncharacterized protein of 272 aas with no strongly hydrophobic peaks that could be TMSs.

UP of Azospirillum picis

Uncharacterized protein of 267 aas with possibly two TMSs between residues 80 and 120.

UP of Geobacter sp.

Gasdermin of 268 aas and possibly one TMS at residue 225. The 3-D structure has been determined (PDB# 7N52_A; Johnson et al. 2022).

Gasdermin of Runella zeae

Uncharacterized protein of 266 aas and possibly 1 or 2 TMSs.

UP of Cytophagia bacterium (microbial mat metagenome)

Uncharacterized protein of 263 aas and 1 TMS at the N-terminus of the protein.

UP of Vibrio campbellii

Uncharacterized protein of 252 aas and 1 N-terminal TMS.

UP of Calditerrivibrio nitroreducens

Uncharacterized protein of 296 aas and 1 N-terminal TMS + two possible TMSs of lesser hydrophobicity.

UP of Planctomycetota bacterium

Uncharacterized protein of 264 aas and possibly 3 TMSs, one N-terminal and two between residues 100 and 145.

UP of Cyclobacteriaceae bacterium (bioreactor metagenome)

Uncharacterized protein of 249 aas and possibly 1 C-terminal TMS.

UP of Bacteroidetes bacterium

Uncharacterized protein of 276 aas and an unknown number of TMSs.

UP of Pirellulaceae bacterium (hot springs metagenome)

Uncharacterized protein of 276 aas and possibly three moderately hydrophobic TMSs at residues 90, 160 and 230.

UP of Bacteroidetes bacterium (phycosphere metagenome)

Uncharacterized protein of 282 aas and possibly 1 C-terminal TMS.

UP of Sphingobacteriales bacterium

Uncharacterized protein of 264 aas and possibly 2 TMSs between residues 10 and 50.  This protein shows sequence similarity between subfamilies 1 and 3 in family 1.C.137.

UP of Chitinophagales bacterium

Uncharacterized protein of 263 aas and 1 or 2 TMSs.

UP of Nannocystis sp. SCPEA4

Uncharacterized protein of 257 aas and possibly 1, 2, or 3 TMSs, one N-terminal and two between residues 75 and 110. This protein shows appreciable sequence similarity with members of TC subfamily 1.C.137.1.

UP of Gemmataceae bacterium

Uncharacterized protein of 216 aas and possibly 1 N-terminal TMS.

UP of Candidatus Eremiobacteraeota bacterium

Uncharacterized protein of 272 aas and possibly 1 TMS at about residue 70.

UP of Pseudomonadota bacterium (soil metagenome)

Uncharacterized protein of 259 aas and possibly 3 TMSs, one N-terminal and two between residues 100 - 140.

UP of Kribbella monticola

Uncharacterized protein of 323 aas with possibly 3 TMSs, one N-terminal, one at residue 80 snf one at residue 160.

UP of Acetobacterium sp.

Uncharacterized protein of 250 aas and possibly 3 TMSs in the N-terminal half of the protein.

UP of Streptomyces sp.