1.C.60 The Two-component Enterococcus faecalis Cytolysin (EFC) Family The EFC family includes a single member, the cytolysin of E. faecalis which consists of two subunits, CylL (large; 68 aas) and CylL (small; 63 aas). They are post-transcriptionally modified by CylM and secreted by the ABC exporter, CylB (TC #3.A.1.111.1). Extracellularly, the two subunits are activated by a protease, CylA yielding the functional mature cytolysin subunits, CylL^"_L and CylL^"_S. CylI confers protection to the producer. The cytolysin is a "pseudohaemolysin" which produces hemolysis on blood agar plates, but not in broth culture. This may be due to regulated synthesis of the cytolysin by an unusual two-component system that senses the extracellular concentration of an inducer via a quorum sensing autoinduction mechanism (Haas et al., 2002). In addition to its toxicity to animal cells, it is bacteriocidal for nearly all Gram-positive bacteria.
This family belongs to the Lantibiotic Bacteriocin (La-Ba) Superfamily.
References:
Haas, W., B.D. Shepard, and M.S. Gilmore. 2002. Two-component regulator of Enterococcus faecalis cytolysin responds to quorum-sensing autoinduction. Nature 415: 84-86.
Hällgren, A., C. Claesson, B. Saeedi, H.J. Monstein, H. Hanberger, and L.E. Nilsson. (2009). Molecular detection of aggregation substance, enterococcal surface protein, and cytolysin genes and in vitro adhesion to urinary catheters of Enterococcus faecalis and E. faecium of clinical origin. Int. J. Med. Microbiol. 299: 323-332.
Kim, S.W., S.I. Kang, D.H. Shin, S.Y. Oh, C.W. Lee, Y. Yang, Y.K. Son, H.S. Yang, B.H. Lee, H.J. An, I.S. Jeong, and W.Y. Bang. (2020). Potential of Cell-Free Supernatant from NIBR97, Including Novel Bacteriocins, as a Natural Alternative to Chemical Disinfectants. Pharmaceuticals (Basel) 13:.
Examples:
TC#	Name	Organismal Type	Example
1.C.60.1.1	Cytolysin, CylL_L/CylL_{S (Hällgren et al. 2009).}	Gram-positive bacteria	*CylL_L and CylL_S of Enterococcus* species**

1.C.60.1.10	Type 2 lantibiotic of 61 aas and 1 TMS		Lantibiotic of Lactobacillus reuteri

1.C.60.1.11	Type 2 lantibiotic, SP_1948 family of 132 aas and 1 TMS.		Lantibiotic of Pilibacter termitis

1.C.60.1.12	Type 2 lantibiotic of 56 aas and 1 TMS		Lantibiotic of Pilibacter termitis

1.C.60.1.13	Type 2 lantibiotic, partial, of 47 aas and 1 TMS		Lantibiotic of Geobacillus yumthangensis

1.C.60.1.14	Type 2 lantibiotic of 60 aas and 1 TMS		Lantibiotic of Ruminococcus gnavus

1.C.60.1.2	Type 2 lantibiotic of 62 aas and 1 TMS		*Lantibiotic of Eubacterium plexicaudatum* ASF492**

1.C.60.1.3	Type 2 lantibiotic of 53 aas and 1 TMS		Lantibiotic of Bacillus thuringiensis

1.C.60.1.4	Mersacidin/lichenicidin family type 2 lantibiotic, partial, of 74 aas and 1 TMS.		Lantibiotic of Bacillus cereus

1.C.60.1.5	Type 2 lantibiotic of 106 aas and 1 TMS		Lantibiotic of Lactobacillus gastricus

1.C.60.1.6	Type 2 lantibiotic, SP_1948 family of 78 aas and 1 TMS		Lantibiotic of Mycobacteroides abscessus

1.C.60.1.7	Type 2 lantibiotic of 70 aas and 1 TMS		*Lantibiotic of an unclassified Actinomyces* specie**

1.C.60.1.8	Type 2 lantibiotic of 69 aas and 1 TMS.		*Lantibiotic of Robinsoniella* sp.**

1.C.60.1.9	Uncharacterized protein of 62 aas and 1 TMS		UP of Paenibacillus zanthoxyli

Examples:
TC#	Name	Organismal Type	Example
1.C.60.2.1	Uncharacterized protein of 71 aas and 1 TMS		UP of Lachnospira pectinoschiza

1.C.60.2.2	Uncharacterized protein of 59 aas and 1 TMS		UP of Planococcus maitriensis

1.C.60.2.3	Uncharacterized protein of 63 aas and 1 TMS.		UP of Lachnospira pectinoschiza

1.C.60.2.4	Class II lanthipeptide, LchA2/BrtA2 family of 60 aas and 1 TMS.		LchA2 of Paenibacillus zeisoli

1.C.60.2.5	Class II lanthipeptide, LchA2/BrtA2 family of 60 aas with 1 TMS.		Lanthipeptide of Microbacterium sp.

Examples:
TC#	Name	Organismal Type	Example
1.C.60.3.1	*Plantaricin C of 82 aas and 1 TMS (Kim et al.* 2020).**		Plantaricin C of Clostridiales bacterium FE2011

1.C.60.3.2	Plantaricin C family lantibiotic of 81 aas and 1 TMS.		Plantaricin C of Clostridiales bacterium FE2011