*1.E.25 The Pseudomonas* phage F116 Holin (F116 Holin) Family** The Pseudomonas phage F116 holin is a non-characterized holin homologous to one in Neisseria gonorrheae that has been characterized (Piekarowicz et al. 2007). Bioinformatic analysis of the genome sequence of N. gonorrhoeae revealed the presence of nine probable prophage islands. The genomic sequence of FA1090 identified five genomic regions (NgoPhi1 - 5) that are related to dsDNA lysogenic phage. The DNA sequences from NgoPhi1, NgoPhi2 and NgoPhi3 contained regions of identity. A region of NgoPhi2 showed high similarity with the Pseudomonas aeruginosa generalized transducing phage F116. NgoPhi1 and NgoPhi2 encode functionally active phages. The holin gene of NgoPhi1 (identical to that encoded by NgoPhi2), when expressed in E. coli, could substitute for the phage lambda S gene (Piekarowicz et al. 2007).
This family belongs to the Holin II Superfamily .
References:
Czajkowski, R. (2019). May the Phage be With You? Prophage-Like Elements in the Genomes of Soft Rot : spp. and spp. Front Microbiol 10: 138.
Piekarowicz, A., A. Kłyz, M. Majchrzak, M. Adamczyk-Popławska, T.K. Maugel, and D.C. Stein. (2007). Characterization of the dsDNA prophage sequences in the genome of Neisseria gonorrhoeae and visualization of productive bacteriophage. BMC Microbiol 7: 66.
Examples:
TC#	Name	Organismal Type	Example
1.E.25.1.1	Pseudomonas phage F116 holin	Phage	*Holin of Pseudomonas* phage F116**

1.E.25.1.2	Variovorax paradoxus holin	Proteobacteria	Holin of Variovorax paradoxus

1.E.25.1.3	hypothetical protein	Proteobacteria	Holin of Bordetella bronchiseptica

1.E.25.1.4	Uncharacterized protein	Proteobacteria	Uncharacterized protein of Acidovorax citrulli

1.E.25.1.5	Putative holin	Proteobacteria	Putative holin of Laribacter hongkongensis

1.E.25.1.7	Holin of 83 aas and 1 TMS		Holin of Acinetobacter nosocomialis

Examples:
TC#	Name	Organismal Type	Example
1.E.25.2.1	Serratia NucE (RegA) holin (Berkmen et al. 1997).	Proteobacteria	*NucE of Serratia marcescens* (gbU11698)**

1.E.25.2.2	Putative holin, NucE	γ-Proteobacteria	NucE of Erwinia tasmaniensis

1.E.25.2.3	Putative holin of 100 aas and 2 N-terminal TMSs		Holin of Pectobacterium atrosepticum

1.E.25.2.4	Putative holin and DUF4254 domain-containing protein of 94 aas and 1 or 2 N-terminal TMSs.		Holin of Raoultella terrigena

1.E.25.2.5	Putative holin of 97 aas and 1 or 2 TMSs.		Holin of Yersinia frederiksenii

1.E.25.2.6	Putative holin of 73 aas and 2 TMSs.		Holin of Pectobacterium zantedeschiae

1.E.25.2.7	Putative holin of 89 aas and 1 or 2 TMSs (Czajkowski 2019).		*Holin of Dickeya* phage BF25/12**

1.E.25.2.8	Phage holinof 79 aas and 2 TMSs.		Holin of Chania multitudinisentens