| 1.E.26 The Holin LLH (Holin LLH) Family The Holin LLH family is found in Firmicutes and phage of Firmicutes as well as other bacteria. Members are fairly large ((100 - 160 aas) and have an N-terminal transmembrane segment. | ||||
This family belongs to the Holin VI Superfamily . | ||||
| References: | ||||
| Gindreau, E. and A. Lonvaud-Funel. (1999). Molecular analysis of the region encoding the lytic system from Oenococcus oeni temperate bacteriophage phi 10MC. FEMS Microbiol. Lett. 171: 231-238. | ||||
| Guo, T., C. Zhang, W. Liu, S. Wang, and J. Kong. (2015). Functional analysis of the N-terminal region of endolysin Lyb5 encoded by Lactobacillus fermentum bacteriophage φPYB5. Int J Food Microbiol 203: 1-7. | ||||
| van der Ploeg, J.R. (2007). Genome sequence of Streptococcus mutans bacteriophage M102. FEMS Microbiol. Lett. 275: 130-138. | ||||
| Wang, S., J. Kong, and X. Zhang. (2008). Identification and characterization of the two-component cell lysis cassette encoded by temperate bacteriophage phiPYB5 of Lactobacillus fermentum. J Appl Microbiol 105: 1939-1944. | ||||
| Yuan, W., Y. Zhang, G. Wang, J. Bai, X. Wang, Y. Li, and P. Jiang. (2016). Genomic and proteomic characterization of SE-I, a temperate bacteriophage infecting Erysipelothrix rhusiopathiae. Arch Virol 161: 3137-3150. | ||||
| Examples: | ||||
| TC# | Name | Organismal Type | Example | |
| 1.E.26.1.1 | Holin (105 aas) | Firmicutes | Holin of Clostridium phytofermentans | |
| 1.E.26.1.10 | Putative holin of 186 aas | Firmicutes | Putative holin of Paenibacillus polymyxa | |
| 1.E.26.1.11 | Uncharacterized protein of 172 aas | Firmicutes | UP of Lactobacillus brevis | |
| 1.E.26.1.12 | Putative holin of 109 aas and 1 TMS | Chloroflexi | Putative holin of Nitrolancea hollandica | |
| 1.E.26.1.13 | Putative holin of 113 aas and 1 TMS | Firmicutes | Putative holin of Paenibacillus polymyxa | |
| 1.E.26.1.14 | Holin of 111 aas and 1 N-terminal TMS. | Holin of Bacillus phage vB_BcoS-136 | ||
| 1.E.26.1.2 | SE-1 phage holin of 123 aas and 1-3 TMSs (Yuan et al. 2016). | Firmicutes | Holiin of SE-1 phage infecting Erysipelothrix rhusiopathiae | |
| 1.E.26.1.3 | Phage holin of the LL0H family (110 aas) | Firmicutes | Holin of Streptococcus pneumoniae | |
| 1.E.26.1.4 | Putative holin of 1112 aas and 1 TMS | Firmicutes | Putative holin of Caldicellulosiruptor owensensis | |
| 1.E.26.1.5 | Putative holin of 109 aas and 2 TMSs | Thermotogae | Putative holin of Marinitoga piezophila | |
| 1.E.26.1.6 | Holin of S. mutans phage M102 (van der Ploeg 2007). | Firmicute viruses | Holin of phage M102 | |
| 1.E.26.1.7 | Putative holin of 109 aas and 1 TMS. | Firmicutes | Putative holin of Caldicellulosiruptor hydrothermalis | |
| 1.E.26.1.8 | Holin_LLH superfamily member | Firmicutes | holin of Desulfotomaculum gibsoniae | |
| 1.E.26.1.9 | Putative holin of 112 aas, Gp072 | Firmicute virus | Gp072 of Lactococcus phage KSY1 | |
| Examples: | ||||
| TC# | Name | Organismal Type | Example | |
| 1.E.26.10.1 | Uncharacterized protein of 107 aas | Tenericutes | UP of Acholeplasma laidlawii | |
| Examples: | ||||
| TC# | Name | Organismal Type | Example | |
| 1.E.26.11.1 | Putative holin of 140 aas and 1 or 2 TMSs | Thermus | Putative holin of Meiothermus ruber | |
| 1.E.26.11.2 | Putative holin of 140 aas and 1 TMS | Thermus | Putative holin of Thermus sp. RL | |
| 1.E.26.11.3 | Holin of Thermus phage P23-77 of 140 aas and 1 TMS | Thermus | Holin of Thermus phage P23-77 | |
| Examples: | ||||
| TC# | Name | Organismal Type | Example | |
| 1.E.26.2.1 | Holin of Lactobacillus phage LF1 | Firmicute viruses | Holin of Lactobacillus phage LF1 | |
| 1.E.26.2.2 | Holin P163 (Gindreau and Lonvaud-Funel 1999). | Firmicute viruses | Holin P163 of Leuconostoc phage 10MC | |
| 1.E.26.2.3 | Putative holin of 117 aas | Firmicute virus | Putative holin of Lactobacillus phage JCL1032 | |
| 1.E.26.2.4 | Uncharacterized protein of 140 aa | Firmicutes | UP of Lactobacillus acidophilus | |
| 1.E.26.2.5 | Holin, Hyb50 of 254 aas. This holin causes lysis by itself, but with the lytic enzyme, Lyb50, lysis occurs more rapidly (Wang et al. 2008). Smaller amounts of Lyb50 can reach the periplasm without the holin (Guo et al. 2015). | Lactobacillus virus | Holin of Lactobacillus phage phiPYB5 | |
| Examples: | ||||
| TC# | Name | Organismal Type | Example | |
| 1.E.26.3.1 | Holin of the LL-H family (105 aas) | Firmicutes | Holin LL-H of Peptoniphilus harei | |
| 1.E.26.3.2 | Uncharacterized protein of 126 aas and 1 TMS | Firmicutes | UP of Clostridium acetobutylicum | |
| 1.E.26.3.3 | Phage-like holin of 127 aas and 1 N-terminal TMS. | Holin of Peptoniphilus indolicus | ||
| 1.E.26.3.4 | Phage holin, LL-H family of 123 aas and 1 TMS. | Holin of Clostridium pasteurianum BC1 | ||
| Examples: | ||||
| TC# | Name | Organismal Type | Example | |
| 1.E.26.4.1 | Putative holin of 153 aas and 1 N-terminal TMS | Firmicutes | Holin of Lactococcus lactis | |
| Examples: | ||||
| TC# | Name | Organismal Type | Example | |
| 1.E.26.5.1 | Putative holin of 118 aas and 1 TMS | Thermus | Putative holin of Thermus phage P74-26 | |
| Examples: | ||||
| TC# | Name | Organismal Type | Example | |
| 1.E.26.6.1 | Uncharacterized protein of 145 aas and 1 TMS | Firmicutes | UP of Clostridium kluyveri | |
| 1.E.26.6.2 | Putative holin of 166 aas | Firmicutes | Putative porin of Desulfosporosinus meridiei | |
| 1.E.26.6.3 | Uncharaccterized protein of 177 aas | Firmicutes | UP of Thermoanaerobacterium saccharolyticum | |
| Examples: | ||||
| TC# | Name | Organismal Type | Example | |
| 1.E.26.7.1 | Uncharacterized protein of 96 aas | Firmicutes | UP of Clostridium acidurici | |
| 1.E.26.7.2 | Putative holin of 162 aas and 1 or 2 TMSs | Fusobacteria | Putative holin of Fusobacterium varium | |
| 1.E.26.7.3 | Putative holin of 162 aas and 1 or 2 TMSs | Fusobacteria | Putative holin of Fusobacterium ulcerans | |
| 1.E.26.7.4 | Putative holin of 167 aas and 1 or 2 TMSs | Fusobacteria | Putative holin of Sebaldella termitidis | |
| 1.E.26.7.5 | Putative holin of 134 aas and 1 TMS | Firmicutes | Putative holin of Faecalibacterium prausnitzii | |
| Examples: | ||||
| TC# | Name | Organismal Type | Example | |
| 1.E.26.8.1 | Uncharacterized protein of 172 aas | Firmicutes | UP of Clostridium pasteurianum | |
| 1.E.26.8.2 | Phage holin of 161 aas and 1 TMS. | Holin of Clostridium phage phiSM101 | ||
| 1.E.26.8.3 | Holin of 139 aas and 1 TMSs. | Holin of Clostridium kluyveri | ||
| Examples: | ||||
| TC# | Name | Organismal Type | Example | |
| 1.E.26.9.1 | Holin of 114 aas | Firmicutes | Holin of Lactobacillus johnsonii | |
| 1.E.26.9.2 | Phage holin of 118 aas with one of two TMSs, one N-terminal and the other (of lesser hydrophobicity) at residue 45. | Phage holin of Lactobacillus sp. | ||