2.A.114 The Peptide Transporter Carbon Starvation CstA (CstA) Family

The CstA family consists of proteins from bacteria, and archaea. These proteins are of various sizes and topologies. For example, CstA of E. coli has 701 aas with 18 putative TMSs. It has a long N-terminal CstA domain and a short C-terminal DUF4161 domain. This protein is encoded by a carbon starvation inducible gene cstA that is under cyclic AMP-CRP control. Circumstantial evidence suggested that it may be a peptide transporter (Schultz and Matin, 1991). A Campylobacter jejuni homologue has been shown to transport di- and tripeptides (see TC# 2.A.114.1.5) (Rasmussen et al., 2013).



This family belongs to the APC Superfamily.

 

References:

Behr, S., L. Fried, and K. Jung. (2014). Identification of a novel nutrient-sensing histidine kinase/response regulator network in Escherichia coli. J. Bacteriol. 196: 2023-2029.

Garai, P., K. Chandra, J. Chatterjee, and D. Chakravortty. (2017). Peptide transporter YjiY influences the expression of the virulence gene mgtC to regulate biofilm formation in Salmonella. FEMS Microbiol. Lett. [Epub: Ahead of Print]

Hwang, S., D. Choe, M. Yoo, S. Cho, S.C. Kim, S. Cho, and B.K. Cho. (2018). Peptide Transporter CstA Imports Pyruvate in Escherichia coli K-12. J. Bacteriol. 200:.

Kraxenberger, T., L. Fried, S. Behr, and K. Jung. (2012). First insights into the unexplored two-component system YehU/YehT in Escherichia coli. J. Bacteriol. 194: 4272-4284.

Kristoficova, I., C. Vilhena, S. Behr, and K. Jung. (2017). BtsT - a novel and specific pyruvate/Hsymporter in. J. Bacteriol. [Epub: Ahead of Print]

Rasmussen, J.J., C.S. Vegge, H. Frøkiær, R.M. Howlett, K.A. Krogfelt, D.J. Kelly, and H. Ingmer. (2013). Campylobacter jejuni carbon starvation protein A (CstA) is involved in peptide utilization, motility and agglutination, and has a role in stimulation of dendritic cells. J. Med. Microbiol. 62: 1135-1143.

Schultz, J.E. and A. Matin. (1991). Molecular and functional characterization of a carbon starvation gene of Escherichia coli. J. Mol. Biol. 218: 129-140.

Examples:

TC#NameOrganismal TypeExample
2.A.114.1.1

Carbon starvation inducible protein CstA (701 aas; 18 TMSs).  This protein has been shown to be a pyruvate uptake system, working together with a small protein of 65 aas, YbdD (Hwang et al. 2018).  It may also transport peptides.

Bacteria and Archaea

CstA of E. coli (P15078)

 
2.A.114.1.10

BtsT (from the German word for pyruvate: """"Brenztraubensäure"""" transporter) or YjiY of 716 aas and 18 TMSs.  It is a high affinity (Km + 16 μM), inducible, specific pyruvate:proton uptake symporter (Kristoficova et al. 2017). Expression of the btsT (yjiY) gene is regulated by the LytS-like histidine kinase, BtsS, a sensor of extracellular pyruvate, together with the LytTR-like response regulator, BtsR (Kristoficova et al. 2017).It may also mediate uptake of specific peptides, thus initiating their metabolism, but this has not been demonstrated directly. It indirectly influences flagellar biosynthesis and virulence. BtsT (YjiY) is required for successful colonization of Salmonella in the mouse gut (Garai et al. 2015). It also influences expression of the mgtC gene to regulate biofilm formation (Garai et al. 2017).

YjiY of Salmonella enterica; subspecies Typhimurium (strain LT2)

 
2.A.114.1.2

Carbon starvation protein, CstA (484 aas; 13 TMSs).

Archaea

CstA of Anaerococcus prevotii (C7RGN6)

 
2.A.114.1.3

CstA (579 aas; 15 TMSs)

Archaea

CstA of Thermococcus kodakaraensis (Q5JIF7)

 
2.A.114.1.4

CstA of 483 aas with 12 or 13 putative TMSs. 

Firmicutes

CstA of Chlostridium perfringens (Q8XME6)

 
2.A.114.1.5

CstA of 703 aas and 18 putative TMSs. In C. jejuni, this protein plays a role in starvation responses and peptide uptake.  A ΔcstA mutant has reduced use of di- and tri-peptides when used as nitrogen sources. The mutant also has reduced motility and agglutination and shows decreased host-pathogen relationships (Rasmussen et al. 2013).

Proteobacteria

CstA of Campylobacter jejuni (Q0P9Y2)

 
2.A.114.1.6

CstA of 511 aas and 14 putative TMSs in a possible 10 4 arrangement.  The last 4 TMSs correspond to the DUF4161 domain in CDD.

Fibrobacteres

CstA of Fibrobacter succinogenes

 
2.A.114.1.7
CstA of 791 aas and 16 TMSs in an apparent 11 + 5 + 1 arrangement.

Actinobacteria

CstA of Bifidobacterium animalis

 
2.A.114.1.8

CstA of 704 aas and 16 TMSs in a 4 + 6 + 6 arrangement.

Planctomycetes

CstA of Singulisphaera acidiphila

 
2.A.114.1.9

High affinity (Km 16 mμM) pyruvate:H+ symporter, BtsT (from "Brenztraubensaure", the German word for pyruvate), formerly YjiY of 716 aas and 16 - 18 TMSs (Kristoficova et al. 2017).  Regulated by Crp as well as the LytS-like histidine sensor kinase, BtsS (YehU) which senses extracellular pyruvate, and the corresponding LytTR-like response regulator, BtsR (YehT).  Although the true inducer is extracellular pyruvate as noted above, it is induced by peptides as cells enter the stationary growth phase, presumably because pyruvate is released from these peptides (Kraxenberger et al. 2012).  BtsT forms a complex with the MFS transporter, YhjX (TC# 2.A.1.11.3) and two sensor kinase/response regulator pairs, YehU/YehT and YdpA/YdpB (Behr et al. 2014).  Both transporters are also posttranscriptionally regulated by CsrA (Behr et al. 2014).

Proteobacteria

BtsT or YjiY of E. coli

 
Examples:

TC#NameOrganismal TypeExample