8.A.120.  The Mitochondrial StAR-related lipid transfer protein (StAR) Family 

StarD7 is anchored onto the outer mitochondrial membrane through its N-terminal TMS, and the C-terminal START domain may extend into the cytoplasm and shuttle phosphatidyl choline between the ER and the outer mitochondrial membrane at  ER-mitochondrion contact sites (Horibata et al. 2017) (see TC# 1.R.1). Therefore, StarD7 facilitates phosphatidylcholine (PC) transfer to mitochondria and is essential for mitochondrial homeostasis. A  putative TMS, C-terminal to the mitochondria-targeting signal (MTS) sequence at the N-terminus of StarD7 may play an important role. The mature form of StarD7 is integrated into and/or associated with the outer leaflet of the outer mitochondrial membrane. The precursor form of StarD7 is cleaved between Met(76) and Ala(77), and Ala(77) and Ala(78) in the TMS to produce the mature form (Horibata et al. 2017).

MLN64 (metastatic lymph node 64) and MENTHO (MLN64 N-terminal homologue) are two late-endosomal proteins that share a conserved region of four TMSs with three short intervening loops called the MENTAL domain (MLN64 N-terminal domain) (Alpy and Tomasetto 2006). This domain mediates MLN64 and MENTHO homo- and hetero-interactions, targets both proteins to late endosomes and binds cholesterol in vivo. In addition to the MENTAL domain, MLN64 contains a cholesterol-specific START domain [StAR (steroidogenic acute regulatory protein)-related lipid transfer domain]. The START domain is a protein module of approx. 210 residues that binds lipids, including sterols, and is present in 15 distinct proteins in mammals. They may function in cholesterol transport. The MENTAL domain might serve to maintain cholesterol at the membrane of late endosomes prior to its shuttle to cytoplasmic acceptor(s) through the START domain (Alpy and Tomasetto 2006).

The human metastatic lymph node 64 (MLN64) protein is a 4 TMS protein, where residues 52-170 in this 445 aa protein are transmembrane. The Drosophila melanogaster Start1 protein is a homologous 4 TMS protein where residues 62-183 in this 583 aa protein are transmembrane. Roth et al. 2004 proposed that Start1 is a cholesterol transporter.MLN64 transport to the late endosome is regulated by binding to the 14-3-3 protein (TC# 8.A.98.1.1)via a non-canonical binding site (Liapis et al. 2012). Steroid hormone biosynthesis in mitochondria has been reviewed (Miller 2013).

The two proteins, Start1 and MLN64, are homologous to the vertebrate cholesterol-binding steroid acute regulatory protein (STAR)-related lipid transfer domain (START). MLN64 is somehow involved in cholesterol trafficking and/or steroid synthesis. Roth et al. 2004 suggested that Start1 plays a role in the regulation of ecdysteroid synthesis, and expression of Start1 depends on ecdysone. These authors suggested that Start1 is a cholesterol transporter since ecdysteroid is synthesized from cholesterol. Another group, Kennedy et al. 2014  showed that MLN64, mediates endosomal cholesterol transport to mitochondria.

START domains are ~210 aa lipid binding domains implicated in intracellular lipid transport, lipid metabolism and cell signaling (Soccio and Breslow 2003). The human and mouse genomes have 15 genes encoding START domains. The x-ray structures of three such proteins have been solved (see Soccio and Breslow 2003 for a review). The membrane specificity of the human cholesterol transfer protein STARD4 has been deefined (Talandashti et al. 2024).  Targeting STARD4/EGFR axis inhibits growth and overcomes lenvatinib resistance in hepatocellular carcinoma in humans (Liu et al. 2025).

 


 

References:

Alpy, F. and C. Tomasetto. (2006). MLN64 and MENTHO, two mediators of endosomal cholesterol transport. Biochem Soc Trans 34: 343-345.

Arunkumar, R., A. Gorusupudi, and P.S. Bernstein. (2020). The macular carotenoids: A biochemical overview. Biochim. Biophys. Acta. Mol. Cell Biol. Lipids 1865: 158617.

Asif, K., L. Memeo, S. Palazzolo, Y. Frión-Herrera, S. Parisi, I. Caligiuri, V. Canzonieri, C. Granchi, T. Tuccinardi, and F. Rizzolio. (2021). STARD3: A Prospective Target for Cancer Therapy. Cancers (Basel) 13:.

Balboa, E., J. Castro, M.J. Pinochet, G.I. Cancino, N. Matías, P.J. Sáez, A. Martínez, A.R. Álvarez, C. Garcia-Ruiz, J.C. Fernandez-Checa, and S. Zanlungo. (2017). MLN64 induces mitochondrial dysfunction associated with increased mitochondrial cholesterol content. Redox Biol 12: 274-284.

Horibata, Y., H. Ando, M. Satou, H. Shimizu, S. Mitsuhashi, Y. Shimizu, M. Itoh, and H. Sugimoto. (2017). Identification of the N-terminal transmembrane domain of StarD7 and its importance for mitochondrial outer membrane localization and phosphatidylcholine transfer. Sci Rep 7: 8793.

Kennedy, B.E., C.T. Madreiter, N. Vishnu, R. Malli, W.F. Graier, and B. Karten. (2014). Adaptations of energy metabolism associated with increased levels of mitochondrial cholesterol in Niemann-Pick type C1-deficient cells. J. Biol. Chem. 289: 16278-16289.

Liapis, A., F.W. Chen, J.P. Davies, R. Wang, and Y.A. Ioannou. (2012). MLN64 transport to the late endosome is regulated by binding to 14-3-3 via a non-canonical binding site. PLoS One 7: e34424.

Liu, M., Y. Liu, J. Zheng, X. An, J. Wen, F. Zhu, J. Jia, D. Guo, and N. Chen. (2025). Targeting STARD4/EGFR axis inhibits growth and overcomes lenvatinib resistance in hepatocellular carcinoma. Genes Dis 12: 101556.

Miller, W.L. (2013). Steroid hormone synthesis in mitochondria. Mol. Cell Endocrinol 379: 62-73.

Rodriguez-Agudo, D., S. Ren, E. Wong, D. Marques, K. Redford, G. Gil, P. Hylemon, and W.M. Pandak. (2008). Intracellular cholesterol transporter StarD4 binds free cholesterol and increases cholesteryl ester formation. J Lipid Res 49: 1409-1419.

Roth, G.E., M.S. Gierl, L. Vollborn, M. Meise, R. Lintermann, and G. Korge. (2004). The Drosophila gene Start1: a putative cholesterol transporter and key regulator of ecdysteroid synthesis. Proc. Natl. Acad. Sci. USA 101: 1601-1606.

Soccio, R.E. and J.L. Breslow. (2003). StAR-related lipid transfer (START) proteins: mediators of intracellular lipid metabolism. J. Biol. Chem. 278: 22183-22186.

Talandashti, R., L. van Ek, C. Gehin, D. Xue, M. Moqadam, A.C. Gavin, and N. Reuter. (2024). Membrane specificity of the human cholesterol transfer protein STARD4. J. Mol. Biol. 436: 168572.

Xie, H. and H. Weinstein. (2025). Recognition of Specific PIP2-subtype Composition Triggers the Allosteric Control Mechanism for Selective Membrane Targeting of Cargo Loading and Release Functions of the Intracellular Sterol Transporter StarD4. J. Mol. Biol. 169157. [Epub: Ahead of Print]

Examples:

TC#NameOrganismal TypeExample
8.A.120.1.1

StarD7 of 370 aas and 2 TMSs.  See family description and (Horibata et al. 2017) for a functional description.

StarD7 of Homo sapiens

 
8.A.120.1.2

Uncharacterized StarD7-like phosphatidyl choline transfer protein, PCTP-like protein, of 267 aas and 0 TMSs, containing a START domain. 

UP of Neolamprologus brichardi

 
8.A.120.1.3

Uncharacterized protein of 311 aas and 0 TMSs.

UP of Trypanosoma theileri

 
8.A.120.1.4

Uncharacterized protein of 217 aas

UP of Acinetobacter baumannii

 
8.A.120.1.5

Uncharacterized protein of 244 aas

UP of Turneriella parva

 
8.A.120.1.6

Uncharacterized polyketide cyclase/dehydrase and lipid transport superfamily protein of 294 aas and 2 N-terminal TMSs. 

UP of Arabidopsis thaliana

 
Examples:

TC#NameOrganismal TypeExample
8.A.120.2.1

The metastatic lymph node-64 (MLN-64; MLN64; STARD3; CAB1) protein mediates endosomal cholesterol transport to mitochondria and the plasma membrane (Kennedy et al. 2014). It has been implicated in toxin-induced resistance. Down-regulation of MLN64 in Niemann-Pick C1 deficient cells decreased mitochondrial cholesterol content, suggesting that MLN64 functions independently of NPC1 (Balboa et al. 2017), and overexpression increases the mitochondrial cholesterol content and decreases the mitochondrial glutathione content, leading to mitochondrial dysfunction (Balboa et al. 2017). STARD3 is a key protein in the cholesterol movement in cancer cells (Asif et al. 2021). STARD3 is also a lutein- and zeaxanthin (carotenoid)-binding protein (Arunkumar et al. 2020).

Animals

MLN-64 of Homo sapiens (Q14849)

 
8.A.120.2.2The Start1 protein (putative cholesterol transporter)AnimalsStart1 protein of Drosophila melanogaster (AAR19767)
 
8.A.120.2.3

STARD3 N-terminal-like protein, STARD3NL (MENTHO) of 234 aas and 3 TMSs.  MLN64 (metastatic lymph node 64) and MENTHO (MLN64 N-terminal homologue) are two late-endosomal proteins that share a conserved region of four transmembrane helices with three short intervening loops called the MENTAL domain (MLN64 N-terminal domain) (Alpy and Tomasetto 2006). This domain mediates MLN64 and MENTHO homo- and hetero-interactions, targets both proteins to late endosomes and binds cholesterol in vivo. In addition to the MENTAL domain, MLN64 contains a cholesterol-specific START domain [StAR (steroidogenic acute regulatory protein)-related lipid transfer domain]. The START domain is a protein module of approx. 210 residues that binds lipids, including sterols, and is present in 15 distinct proteins in mammals. Thus MLN64 and MENTHO define discrete cholesterol-containing subdomains within the membrane of late endosomes where they may function in cholesterol transport. The MENTAL domain might serve to maintain cholesterol at the membrane of late endosomes prior to its shuttle to cytoplasmic acceptor(s) through the START domain (Alpy and Tomasetto 2006).

STARD3NL of Homo sapiens

 
Examples:

TC#NameOrganismal TypeExample
8.A.120.3.1

STAR-related lipid transfer protein, STARD4, of 205 aas and 0 TMSs.  It is involved in the intracellular transport of cholesterol; it binds cholesterol or other sterols (Rodriguez-Agudo et al. 2008). Xie and Weinstein 2025 presented a quantitative model of the allosteric molecular mechanisms of selective cholesterol (CHL) uptake and delivery by the StarD4 protein - an intracellular cholesterol trafficking protein that facilitates the crucial non-vesicular sterol transport between the plasma membrane and the endoplasmic reticulum. This sterol-specific transfer protein is essential for maintaining the healthy life of human cells. In its physiological function, StarD4 targets both sterol donor and acceptor membranes via interactions with anionic lipids. Experiments have illuminated the kinetics of this sterol transfer and showed it to be modulated by specific phosphatidylinositol phosphates (PIPs) on the target membrane.  The specific molecular mechanism for recognizing PIP2-subtypes in membranes by StarD4 couples to the defined allosteric pathway that induces the CHL binding pocket to propagate the signal for either uptake or release of the sterol. The central role determined for allostery in these significant advances in the understanding of intracellular cholesterol trafficking by StarD4, aligns with experimentally determined properties of StarD4 function, and interprets them in experimentally testable atomistic terms that explain function-altering results of mutations (Xie and Weinstein 2025).

STARD4 of Homo sapiens

 
8.A.120.3.2

StAR related lipid transfer domain containing protein 4, StAR4, of 218 aas and 0 TMSs.

STAR4 of Pelodiscus sinensis (Chinese softshell turtle) (Trionyx sinensis)