9.B.109 The Putative Archaeal 2 TMS Holin (A2-Hol) Family
The archaeon, Candidatus Nitrosoarchaeum limnia, encodes adjacent genes designated Toxin Secretion/Lysis Holin. The 'toxin' gene encodes a soluble protein of 325 aas stated as belonging to the 'Glycosyltransferase GBT-type Superfamily'. This protein brings up other glycosyltransferases in NCBI BLAST searches. The adjacent gene encodes as small protein of 132 aas and 2 TMSs that could be a holin, based on its size and topology, but it could also be a 2 TMS counterpart to the other 2 TMS protein of a two-comoponent transport system. This is suggested because several members of the family have 4 TMSs instead of 2 TMSs. The first protein has the UniProt accession number of S2E3C4 and has been assigned TC# 9.B.109.1.1. Paralogues are found in this same organism and other closely related species. This TC family (9.B.109) appears to be related to TC family 9.B.116 which shows significant sequence similarity with members of 9.B.109 and has the same topology with members having either 2 or 4 TMSs.
Putative holin of 132 aas and 2 TMSs.
Thaumarchaeota
Putative holin of Candidatus Nitrosoarchaeum limnia
Putative holin of 157 aas and 2 TMSs
Thaumarchaeota
Putative holin of Candidatus Nitrosoarchaeum limnia
Putative holin of 165 aas and 2 TMSs
Thaumarchaeota
Putative holin of Candidatus Nitrosoarchaeum koreensis
Putative holin of 121 aas and 2 TMSs.
UP of Nitrosopumilus sp. Nsub
Putative holin of 179 aas and 2 TMSs
UP of uncultured marine thaumarchaeote SAT1000_07_E05
Uncharacterized protein of 202 aas and 2 TMSs
UP of Ignicoccus islandicus
Uncharacterized protein of 144 aas and 2 TMSs.
UP of Candidatus Heimdallarchaeota archaeon B3_Heim (marine sediment metagenome)
Uncharacterized protein of 115 aas and 2 adjacent N-terminal TMSs.
UP of Archaeoglobales archaeon (marine sediment metagenome)
Uncharacterized protein of 170 aas and 2 N-terminal TMSs.
UP of Ignicoccus islandicus