9.B.179 The MscS/DUF475 (DUF475) Family The DUF475 family consists of proteins primarily from Actinobacteria but possibly also Firmicutes. They have 4 N-terminal TMSs that may be related to MARVEL domain containing proteins (TC# 1.A.64) and pantophysins (TC# 9.B.130). The hydrophilic C-terminal domain is G/Q/P-rich and redundant. The 34 kDa antigenic protein homolog, Rv0954, is a protein of 303 aas and 4 TMSs near the N-terminus of the protein. This is one of several dominant glycoproteins that play roles in M. tuberculosis survival, and immunogenicity. They are glycoproteins involved in antigenicity and transport. They may play a role in athogen-host interactions and drug efflux pumps, which are considered as a feasible drug targets or TB new vaccine candidates (Yari et al. 2023).
This family belongs to the Tetraspan Junctional Complex Protein or MARVEL (4JC) Superfamily.
References:
Yari, S., P. Afrough, F. Yari, M. Ghazanfari Jajin, A. Fateh, and A. Hadizadeh Tasbiti. (2023). A potent subset of Mycobacterium tuberculosis glycoproteins as relevant candidates for vaccine and therapeutic target. Sci Rep 13: 22194.
Examples:
TC#	Name	Organismal Type	Example
9.B.179.1.1	Uncharacterized protein of 289 aas and 4 TMSs	Actinobacteria	UP of Streptomyces coelicolor

9.B.179.1.2	DUF475/MscS related uncharacterized protein of 272 aas and 4 N-terminal TMSs.	Actinobacteria	UP of Thermomonospora curvata

9.B.179.1.3	Uncharacterized protein of 336 aas and 4 TMSs	Firmicutes	UP of Streptococcus australis

9.B.179.1.4	Uncharacterized protein of 300 aas and 4 N-terminal TMSs	Actinobacteria	*UP of Frankia* sp.**

9.B.179.1.5	*The tuberculosis potential drug target/vaccine candidate, Rv0954, DUF5336, of 303 aas with 4 TMSs near the N-terminus of the protein (Yari et al.* 2023).**		Rv0954 of Mycobacterium tuberculosis

9.B.179.1.6	DUF5336 domain-containing protein of 387 aas and 4 TMSs.		DUF5336 protein of Tsukamurella asaccharolytica