9.B.32 The DUF3302 or Pfam11742 (YibI) Family The proteins of the DUF3302 family have two N-terminal TMSs followed by a ~50aa C-terminal hydrophilic region. The topology of YihI is established (Daley et al., 2005). These proteins have a well conserved GLPGR motif that may be involved in ATP/GTP binding or hydrolysis.
References:
Daley, D.O., M. Rapp, E. Granseth, K. Melén, D. Drew, and G. von Heijne. (2005). Global topology analysis of the Escherichia coli inner membrane proteome. Science 308: 1321-1323.
Zhu, N., S. Sun, F. Leng, W. Fan, J. Chen, J. Ma, H. He, G. Yang, and Y. Wang. (2022). Identification of Key Genes during Ca-Induced Genetic Transformation in by Combining Multi-Omics and Gene Knockout Techniques. Appl. Environ. Microbiol. 88: e0058722.
Examples:
TC#	Name	Organismal Type	Example
9.B.32.1.1	*DUF3302 (Pfam11742) protein; YibI (120aas, 2 TMSs; Daley et al., 2005).*	Bacteria	*YibI of E. coli* (P32108)**

9.B.32.1.2	YiaW of 107 aas and 2 central TMSs. Upon Ca²⁺ exposure for transformation of DNA plasmids, the level of this protein expressed increases and the amount of plasmid taken up also increases (Zhu et al. 2022).	Bacteria	*YiaW of E. coli* (P0ADK5)**

9.B.32.1.3	Putative GTPase	Bacteria	*Putative GTPase of Vibrio parahaemolyticus* (B8K566)**

9.B.32.1.4	DNA repair ATPase	Bacteria	*ATPase of Aeromonas veronii* (F4DGW5)**

9.B.32.1.5	Hypothetical protein	Bacteria	*HP of Labrenzia alexandrii* (B9QYG9)**

9.B.32.1.6	Hypothetical protein (136aas; 2 TMSs)	Bacteria	*HP of Ralstonia eutropha* (Q0K0R7)**

Examples:
TC#	Name	Organismal Type	Example
9.B.32.2.1	Hypothetical protein (140aas; 2 TMSs) (has a GLPGR motif)	Bacteria	*HP of Candidatus Accumulibacter phosphatis* clade IIA (C7RSB7)**