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1.B.17.1.1
TolC outer membrane exporter of hemolysin, drugs, siderophores such as enterobactin, etc. (Bleuel et al., 2005). The 3-d structure is available (PDB#1EK9). The three monomers form a continuous channel, and each monomer contributes 4 β-strands to the 12 stranded β-barrel (Koronakis et al. 2000). The Salmonella enterica subspecies Typhi homologue is the ST50 antigen (G4C2H4) used in tests for typhoid fever, and a 2.98 Å resolution structure revealed a trimer that forms an alpha-helical tunnel and a beta-barrel transmembrane channel traversing the periplasmic space and outer membrane, respectively (Guan et al. 2015).

Accession Number:P02930
Protein Name:TolC aka MTCB aka MUKA aka REFI aka B3035
Length:493
Molecular Weight:53741.00
Species:Escherichia coli [83333]
Location1 / Topology2 / Orientation3: Cell outer membrane1 / Multi-pass membrane protein2
Substrate Hemolysin, drugs, Siderophores

Cross database links:

Genevestigator: P02930
EchoBASE: EB1002
EcoGene: EG11009
eggNOG: COG1538
HEGENOM: HBG647495
DIP: DIP-11007N
RefSeq: AP_003585.1    NP_417507.2   
Entrez Gene ID: 947521   
Pfam: PF02321   
Drugbank: Drugbank Link   
BioCyc: EcoCyc:EG11009-MONOMER    ECOL168927:B3035-MONOMER   
KEGG: ecj:JW5503    eco:b3035   

Gene Ontology

GO:0009279 C:cell outer membrane
GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005215 F:transporter activity
GO:0042930 P:enterobactin transport
GO:0015031 P:protein transport
GO:0014070 P:response to organic cyclic substance

References (9)

[1] “Primary structure of the tolC gene that codes for an outer membrane protein of Escherichia coli K12.”  Hackett J.et.al.   6312426
[2] “Nucleotide sequence of the tolC gene of Escherichia coli.”  Niki H.et.al.   2216730
[3] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[4] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[5] “The TolC protein of Escherichia coli K12 is synthesised in a precursor form.”  Hackett J.et.al.   6303857
[6] “Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.”  Link A.J.et.al.   9298646
[7] “Extraction of membrane proteins by differential solubilization for separation using two-dimensional gel electrophoresis.”  Molloy M.P.et.al.   9629924
[8] “Protein complexes of the Escherichia coli cell envelope.”  Stenberg F.et.al.   16079137
[9] “Crystal structure of the bacterial membrane protein TolC central to multidrug efflux and protein export.”  Koronakis V.et.al.   10879525
Structure:
1EK9   1TQQ   2VDD   2VDE   2WMZ   2XMN     

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MKKLLPILIG LSLSGFSSLS QAENLMQVYQ QARLSNPELR KSAADRDAAF EKINEARSPL 
61:	LPQLGLGADY TYSNGYRDAN GINSNATSAS LQLTQSIFDM SKWRALTLQE KAAGIQDVTY 
121:	QTDQQTLILN TATAYFNVLN AIDVLSYTQA QKEAIYRQLD QTTQRFNVGL VAITDVQNAR 
181:	AQYDTVLANE VTARNNLDNA VEQLRQITGN YYPELAALNV ENFKTDKPQP VNALLKEAEK 
241:	RNLSLLQARL SQDLAREQIR QAQDGHLPTL DLTASTGISD TSYSGSKTRG AAGTQYDDSN 
301:	MGQNKVGLSF SLPIYQGGMV NSQVKQAQYN FVGASEQLES AHRSVVQTVR SSFNNINASI 
361:	SSINAYKQAV VSAQSSLDAM EAGYSVGTRT IVDVLDATTT LYNAKQELAN ARYNYLINQL 
421:	NIKSALGTLN EQDLLALNNA LSKPVSTNPE NVAPQTPEQN AIADGYAPDS PAPVVQQTSA 
481:	RTTTSNGHNP FRN