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2.A.49.2.2
Intracellular (endosomal) outward rectifying kidney Cl-:H (2:1) antiporter ClC5; CLC-5; CLCN5 (nitrate > Cl- = Br- > I- > acetate > gluconate) (Picollo and Pusch, 2005; Scheel et al., 2005); responsible for Dent''s disease in humans, an X-linked disorder characterized by low molecular weight proteinuria, hypercalciuria, and nephrolithiasis (Stechman et al., 2007; Zifarelli and Pusch, 2009; Ashida et al. 2012). Plays a role by facilitating endosomal acidification through neutralization of proton pump currents (Novarino et al., 2010; Rickheit et al., 2010). The carrier is regulated by protons as studied by Picollo et al., (2010). CLC-5 and KIF3B interact to facilitate CLC-5 plasma membrane expression, endocytosis, and microtubular transport (Reed et al., 2010).  A point mutations can convert NHE-6 into a nitrate (NO3-):H+ antiporter (Zifarelli and Pusch 2009).

Accession Number:Q9GKE7
Protein Name:Outwardly rectifying chloride channel aka CLC5
Length:746
Molecular Weight:83124.00
Species:Sus scrofa (Pig) [9823]
Number of TMSs:11
Location1 / Topology2 / Orientation3: Golgi apparatus membrane1 / Multi-pass membrane protein2
Substrate chloride, bromide, iodide, acetate, gluconate, nitrate

Cross database links:

RefSeq: NP_999304.1   
Entrez Gene ID: 397263   
Pfam: PF00571    PF00654   
KEGG: ssc:397263   

Gene Ontology

GO:0010008 C:endosome membrane
GO:0000139 C:Golgi membrane
GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015297 F:antiporter activity
GO:0005524 F:ATP binding
GO:0005247 F:voltage-gated chloride channel activity
GO:0006821 P:chloride transport
GO:0006897 P:endocytosis
GO:0055085 P:transmembrane transport

References (1)

[1] “Molecular cloning and characterization of an intracellular chloride channel in the proximal tubule cell line, LLC-PK1.”  Dowland L.K.et.al.   10978325

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence 
1:	MDFLEEPIPG VGTYDDFNTI DWVREKSRDR DRHREITNKS KESTWALIHS VSDAFSGWLL 
61:	MLLIGLLSGS LAGLIDISAH WMTDLKEGIC TEGLWFNHEH CCWNSKHVTF KDRDKCPEWN 
121:	SWSQLIISAD EGAFAYIVNY FMYVLWALLF AFLAVSLVKV FAPYACGSGI PEIKTILSGF 
181:	IIRGYLGKWT LIIKTITLVL AVSSGLSLGK EGPLVHVACC CGNILCHCFN KYRKNEAKRR 
241:	EVLSAAAAAG VSVAFGAPIG GVLFSLEEVS YYFPLKTLWR SFFAALVAAF TLRSINPFGN 
301:	SRLVLFYVEF HTPWHLFELV PFILLGIFGG LWGALFIRTN IAWCRKRKTT QLGKYPVIEV 
361:	LVVTAITAIL AFPNEYTRMS TSELISELFN DCGLLDSSKL CDYENRFNTS KAAELPDRPA 
421:	GAGVYSAMWQ LALTLILKIV ITIFTFGMKI PSGLFIPSMA VGAIAGRLLG VGMEQLAYYH 
481:	HDWAIFNSWC SQGADCITPG LYAMVGAAAC LGGVTRMTVS LVVIMFELTG GLEYIVPLMA 
541:	AAMTSKWVAD ALGREGIYDA HIRLNGYPFL EAKEEFAHKT LAMDVMKPRR NDPSLTVLTQ 
601:	DSMTVEDVET IISETTYSGF PVVVSRESQR LVGFVLRRDL IISIENARKK QDGVVSTSII 
661:	YFTEHSPPMP PYTPPTLKLR NILDLSPFTV TDLTPMEIVV DIFRKLGLRQ CLVTHNGRLL 
721:	GIITKKDVLK HIAQMANQDP DSILFN