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Transporter Information:
Name: ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H
Symbol: ATP6V1H
TC: 3.A.2.2.3
Locations: 8p22-q22.3
Aliases: CGI-11, SFD, VMA13, SFDalpha, SFDbeta
GenBank: AF132945
Swiss-Prot: Q9UI12
Accession Number: NM_015941
Old Name: ATPase, H+ transporting, lysosomal 50/57kD, V1 subunit H
PubMed (9620685): Lu X, Yu H, Liu SH, Brodsky FM, Peterlin BM. Interactions between HIV1 Nef and vacuolar ATPase facilitate theinternalization of CD4.Immunity. 1998 May;8(5):647-56. PMID: 9620685 [PubMed - indexed for MEDLINE]

CD4 is the primary receptor for the human immunodeficiency virus (HIV). Nef is an accessory protein of HIV that decreases the expression of CD4 on the surface of infected cells. In this study, we identified the Nef binding protein 1 (NBP1), which interacts specifically with Nef in vitro and in vivo. Since it shares sequence similarity with the catalytic subunit of the vacuolar ATPase (V-ATPase) and complements the loss of this VMA13 gene in yeast, NBP1 is the human homolog of Vma13p. Direct interactions between Nef and NBP1 were correlated with the ability of Nef to internalize CD4. The expression of the antisense NBP1 abrogated these effects. We conclude that NBP1 helps to connect Nef with the endocytic pathway.

PubMed (10810093): Lai CH, Chou CY, Ch'ang LY, Liu CS, Lin W. Identification of novel human genes evolutionarily conserved in Caenorhabditiselegans by comparative proteomics.Genome Res. 2000 May;10(5):703-13. PMID: 10810093 [PubMed - indexed for MEDLINE]

Modern biomedical research greatly benefits from large-scale genome-sequencing projects ranging from studies of viruses, bacteria, and yeast to multicellular organisms, like Caenorhabditis elegans. Comparative genomic studies offer a vast array of prospects for identification and functional annotation of human ortholog genes. We presented a novel comparative proteomic approach for assembling human gene contigs and assisting gene discovery. The C. elegans proteome was used as an alignment template to assist in novel human gene identification from human EST nucleotide databases. Among the available 18,452 C. elegans protein sequences, our results indicate that at least 83% (15,344 sequences) of C. elegans proteome has human homologous genes, with 7,954 records of C. elegans proteins matching known human gene transcripts. Only 11% or less of C. elegans proteome contains nematode-specific genes. We found that the remaining 7,390 sequences might lead to discoveries of novel human genes, and over 150 putative full-length human gene transcripts were assembled upon further database analyses. [The sequence data described in this paper have been submitted to the

>Q9UI12|VATH_HUMAN Vacuolar ATP synthase subunit H - Homo sapiens (Human).