3.A.1.7.3
High-affinity phosphate-specific permease, PstAB/PhoS. The 3-d structure of PhoS = (PBP) = PfluDING) has been solved at high resolution by x-ray crystallography (Ahn et al. 2007) with phosphate bound (4F1U and 4F1V; 0.95Å resolution) and with arsenate bound (4F18 and 4F19; 0.88Å resolution) (Elias et al. 2012). Phosphate binds with 500-fold higher affinity than arsenate due to a dense and rigid network of ion-dipole interactions (Elias et al. 2012). The PBP from Halomonas sp. GFAJ-1 has a phosphate affinity 5000-fold higher than that of arsenate (Elias et al. 2012).
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| Accession Number: | C3KCB6 |
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| Protein Name: | Phosphate transport system permease protein |
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| Length: | 297 |
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| Molecular Weight: | 31288.00 |
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| Species: | Pseudomonas fluorescens (strain SBW25) [216595] |
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| Number of TMSs: | 7 |
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| Location1 / Topology2 / Orientation3: |
Membrane1 / Multi-pass membrane protein2 |
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| Substrate |
phosphate(3-) |
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1: MTDLSSPITA MPSLQRRFEG RALRSLVLTT LVWFGALLAS VPLISVLYML ITRGGARLSL
61: EVFTELPPTG FETGGGFGNA MAGTFVMVGI AAAIAVPVGI MAAIFLAELG PDSKLANASR
121: FAAKMLTGLP SILAGVFAYA LVVMTTGTYS APAGGVALAV LMLPIVVLTA EESMKMVPKI
181: MKDAAYGMGC TRSQVIWKIV LPTGMPAILT GVMLAVARAA GETAPLLFTA LFSNYWIYHD
241: GSLAVMNPTA SLAVLIYNFS GMPFDNQLEL AWAASLVLVM IVLVVNIVSR IFGKPKY