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3.A.1.3.10
Cystine/cysteine/diaminopimelate transporter, CysXYZ; these proteins are also designated FliY/YecS/YecC.  Note, another transporter is designated CysZ in E. coli (TC# 2.A.121.1.1).  CysXYZ also transports the toxic amino acid analogues, L-selenaproline (SCA; L-selenazolidine-4-carboxylic acid) and L-selenocystine (SeCys) (Deutch et al. 2014). FliY binds L-cystine, L-cysteine, and D-cysteine with micromolar affinities, but binding of the L- and D-enantiomers induced different conformational changes in FliY, where the L- enantiomer/SBP complex interacted more efficiently with the YecSC transporter. YecSC has low basal ATPase activity that is moderately stimulated by apo-FliY, more strongly by D-cysteine-bound FliY, and maximally by L-cysteine- or L-cystine-bound FliY (Sabrialabe et al. 2020). FliY may exist in a conformational equilibrium between an open, unliganded form that does not bind to the YecSC transporter and closed, unliganded and closed, liganded forms that bind this transporter with variable affinities but equally stimulate its ATPase activity. These findings differ from previous observations for similar ABC transporters, highlighting the extent of mechanistic diversity in this large protein family (Sabrialabe et al. 2020).

Accession Number:P0AFT2
Protein Name:Inner membrane amino-acid ABC transporter permease protein YECS CysY aka B1918
Length:222
Molecular Weight:24801.00
Species:Escherichia coli [83333]
Number of TMSs:5
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate DAP, Cystine

Cross database links:

RefSeq: AP_002533.1    NP_416428.1   
Entrez Gene ID: 949105   
Pfam: PF00528   
BioCyc: EcoCyc:G7037-MONOMER    ECOL168927:B1918-MONOMER   
KEGG: ecj:JW1903    eco:b1918   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005215 F:transporter activity
GO:0006865 P:amino acid transport

References (4)

[1] “A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map.”  Itoh T.et.al.   9097040
[2] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[3] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[4] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MQESIQLVID SLPFLLKGAG YTLQLSIGGM FFGLLLGFIL ALMRLSPIWP VRWLARFYIS 
61:	IFRGTPLIAQ LFMIYYGLPQ FGIELDPIPS AMIGLSLNTA AYAAETLRAA ISSIDKGQWE 
121:	AAASIGMTPW QTMRRAILPQ AARVALPPLS NSFISLVKDT SLAATIQVPE LFRQAQLITS 
181:	RTLEVFTMYL AASLIYWIMA TVLSTLQNHF ENQLNRQERE PK