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3.D.4.4.1
Cytochrome oxidase including heme A synthase (HAS) and 4 subunits of the cytochrome oxidase.  The 3-d structure of heme A synthase (of 306 aas and 8 TMSs) at 2.2 Å resolution has been solved revealing that the N- and C-terminal halves of HAS consist of four-helix bundles and they align in a pseudo twofold symmetry manner. Each bundle contains a pair of histidine residues and forms a heme-binding domain. The C-half domain binds a cofactor-heme molecule, while the N-half domain is vacant (Niwa et al. 2018). The Sco1 protein, YpmQ, is an accessory protein involved in the assembly of cytochrome c oxidase (Andrews et al. 2004).

Accession Number:P12946
Protein Name:Heme A synthase
Length:306
Molecular Weight:34085.00
Species:Bacillus subtilis [1423]
Number of TMSs:8
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate hydron

Cross database links:

Entrez Gene ID: 937030   
Pfam: PF02628   
KEGG: bsu:BSU14870   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0016627 F:oxidoreductase activity, acting on the CH-CH group of donors
GO:0005515 F:protein binding
GO:0006784 P:heme a biosynthetic process
GO:0030435 P:sporulation resulting in formation of a cellular spore

References (6)

[1] “Isolation and sequence of ctaA, a gene required for cytochrome aa3 biosynthesis and sporulation in Bacillus subtilis.”  Mueller J.P.et.al.   2549006
[2] “The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.”  Kunst F.et.al.   9384377
[3] “Bacillus subtilis ctaA is a heme-containing membrane protein involved in heme A biosynthesis.”  Svensson B.et.al.   7961419
[4] “Identification of novel hemes generated by heme A synthase: evidence for two successive monooxygenase reactions.”  Brown K.R.et.al.   12206660
[5] “Heme O synthase and heme A synthase from Bacillus subtilis and Rhodobacter sphaeroides interact in Escherichia coli.”  Brown B.M.et.al.   15491161
[6] “Heme A synthase enzyme functions dissected by mutagenesis of Bacillus subtilis ctaA.”  Hederstedt L.et.al.   16321940
Structure:
6A2J   6IED     

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence 
1:	MNKALKALGV LTTFVMLIVL IGGALVTKTG SGQGCGRQWP LCHGRFFPEL NPASIIEWSH 
61:	RFASGISIIL VLSLAFWSWR KITPIFRETT FLAIMSIIFL FLQALLGALA VVFGSNALIM 
121:	ALHFGISLIS FASVLILTLL IFEADKSVRT LVKPLQIGKK MQFHMIGILI YSYIVVYTGA 
181:	YVRHTESSLA CPNVPLCSPL NNGLPTQFHE WVQMGHRAAA LLLFVWIIVA AVHAITSYKD 
241:	QKQIFWGWIS CLIFITLQAL SGIMIVYSEL ALGFALAHSF FIACLFGVLC YFLLLIARFR 
301:	YESRQS