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3.A.1.3.10
Cystine/cysteine/diaminopimelate transporter, CysXYZ; these proteins are also designated FliY/YecS/YecC.  Note, another transporter is designated CysZ in E. coli (TC# 2.A.121.1.1).  CysXYZ also transports the toxic amino acid analogues, L-selenaproline (SCA; L-selenazolidine-4-carboxylic acid) and L-selenocystine (SeCys) (Deutch et al. 2014). FliY binds L-cystine, L-cysteine, and D-cysteine with micromolar affinities, but binding of the L- and D-enantiomers induced different conformational changes in FliY, where the L- enantiomer/SBP complex interacted more efficiently with the YecSC transporter. YecSC has low basal ATPase activity that is moderately stimulated by apo-FliY, more strongly by D-cysteine-bound FliY, and maximally by L-cysteine- or L-cystine-bound FliY (Sabrialabe et al. 2020). FliY may exist in a conformational equilibrium between an open, unliganded form that does not bind to the YecSC transporter and closed, unliganded and closed, liganded forms that bind this transporter with variable affinities but equally stimulate its ATPase activity. These findings differ from previous observations for similar ABC transporters, highlighting the extent of mechanistic diversity in this large protein family (Sabrialabe et al. 2020).

Accession Number:P37774
Protein Name:YECC aka CysZ aka B1917
Length:250
Molecular Weight:27677.00
Species:Escherichia coli [83333]
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Peripheral membrane protein2
Substrate DAP, Cystine

Cross database links:

DIP: DIP-11822N
RefSeq: AP_002532.1    NP_416427.1   
Entrez Gene ID: 946422   
Pfam: PF00005   
BioCyc: EcoCyc:EG12347-MONOMER    ECOL168927:B1917-MONOMER   
KEGG: ecj:JW1902    eco:b1917   

Gene Ontology

GO:0005886 C:plasma membrane
GO:0005524 F:ATP binding
GO:0016887 F:ATPase activity
GO:0006865 P:amino acid transport

References (5)

[1] “A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map.”  Itoh T.et.al.   9097040
[2] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[3] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[4] “Multiple control elements for the uvrC gene unit of Escherichia coli.”  Sharma S.et.al.   3515318
[5] “Intrinsic and extrinsic approaches for detecting genes in a bacterial genome.”  Borodovsky M.et.al.   7984428

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FASTA formatted sequence
1:	MSAIEVKNLV KKFHGQTVLH GIDLEVKPGE VVAIIGPSGS GKTTLLRSIN LLEQPEAGTI 
61:	TVGDITIDTA RSLSQQKSLI RQLRQHVGFV FQNFNLFPHR TVLENIIEGP VIVKGEPKEE 
121:	ATARARELLA KVGLAGKETS YPRRLSGGQQ QRVAIARALA MRPEVILFDE PTSALDPELV 
181:	GEVLNTIRQL AQEKRTMVIV THEMSFARDV ADRAIFMDQG RIVEQGAAKA LFADPEQPRT 
241:	RQFLEKFLLQ