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2.A.6.2.6
Multiple drug; N-(3-oxododecanoyl)- L-homoserine lactone autoinducer efflux pump, MexB (functions with MexA (an MFP, 8.A.1) and OprM (an OMF, 1.B.17; see 2.A.6.2.21). All three interact with each other. MexA promotes assembly and stability of the complex (Nehme and Poole, 2007)). Exports β-lactams, fluoroquinolones, tetracycline, macrolides, chloramphenicol, biocides, and a toxic indole compound, CBR-4830, that targets the MreB actin (Robertson et al., 2007). Confers tolerance to tea tree oil and its monoterpene components Terpinen-4-ol, 1,8-cineole and α-terpineol (Papadopoulos et al., 2008) as well as the antimicrobial peptide, colistin (Pamp et al., 2008) (Mao et al., 2002; Poole, 2008). The crystal structure has been solved at 3.0Å resolution (Sennhauser et al., 2009). The MexA-OprM complex has an elongated cylindrical appearance (Trépout et al., 2010).  Mutations affecting export of antibiotics with cytoplasmic targets have been identified (Ohene-Agyei et al. 2012). RND-type xenobiotic transporters recognize hydrophobic substrates such as organic solvents by their periplasmic domains and expel them to the external milieu (Li et al. 2006). Differential impacts of MexB mutations on substrate selectivity of the MexAB-OprM multidrug efflux pump have been reported (Middlemiss and Poole 2004).

Accession Number:P52002
Protein Name:MexB aka PA0426
Length:1046
Molecular Weight:112791.00
Species:Pseudomonas aeruginosa [287]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate

Cross database links:

RefSeq: NP_249117.1   
Entrez Gene ID: 877852   
Pfam: PF00873   
BioCyc: PAER208964:PA0426-MONOMER   
KEGG: pae:PA0426   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005215 F:transporter activity
GO:0046677 P:response to antibiotic
GO:0006810 P:transport

References (8)

[1] “Cloning and sequence analysis of an EnvCD homologue in Pseudomonas aeruginosa: regulation by iron and possible involvement in the secretion of the siderophore pyoverdine.”  Poole K.et.al.   7968531
[2] “Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen.”  Stover C.K.et.al.   10984043
[3] “Molecular mechanisms of bacterial virulence elucidated using a Pseudomonas aeruginosa-Caenorhabditis elegans pathogenesis model.”  Mahajan-Miklos S.et.al.   9989496
[4] “Global analysis of the membrane subproteome of Pseudomonas aeruginosa using liquid chromatography-tandem mass spectrometry.”  Blonder J.et.al.   15253424
[5] “Multiple antibiotic resistance in Pseudomonas aeruginosa: evidence for involvement of an efflux operon.”  Poole K.et.al.   8226684
[6] “Role of mexA-mexB-oprM in antibiotic efflux in Pseudomonas aeruginosa.”  Li X.-Z.et.al.   8540696
[7] “Role of the multidrug efflux systems of Pseudomonas aeruginosa in organic solvent tolerance.”  Li X.-Z.et.al.   9603892
[8] “Membrane topology of the xenobiotic-exporting subunit, MexB, of the MexA,B-OprM extrusion pump in Pseudomonas aeruginosa.”  Guan L.et.al.   10187844
Structure:
2V50   3W9I   3W9J   6IIA   6IOK   6IOL     

External Searches:

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSKFFIDRPI FAWVIALVIM LAGGLSILSL PVNQYPAIAP PAIAVQVSYP GASAETVQDT 
61:	VVQVIEQQMN GIDNLRYISS ESNSDGSMTI TVTFEQGTDP DIAQVQVQNK LQLATPLLPQ 
121:	EVQRQGIRVT KAVKNFLMVV GVVSTDGSMT KEDLSNYIVS NIQDPLSRTK GVGDFQVFGS 
181:	QYSMRIWLDP AKLNSYQLTP GDVSSAIQAQ NVQISSGQLG GLPAVKGQQL NATIIGKTRL 
241:	QTAEQFENIL LKVNPDGSQV RLKDVADVGL GGQDYSINAQ FNGSPASGIA IKLATGANAL 
301:	DTAKAIRQTI ANLEPFMPQG MKVVYPYDTT PVVSASIHEV VKTLGEAILL VFLVMYLFLQ 
361:	NFRATLIPTI AVPVVLLGTF GVLAAFGFSI NTLTMFGMVL AIGLLVDDAI VVVENVERVM 
421:	AEEGLSPREA ARKSMGQIQG ALVGIAMVLS AVFLPMAFFG GSTGVIYRQF SITIVSAMAL 
481:	SVIVALILTP ALCATMLKPI EKGDHGEHKG GFFGWFNRMF LSTTHGYERG VASILKHRAP 
541:	YLLIYVVIVA GMIWMFTRIP TAFLPDEDQG VLFAQVQTPP GSSAERTQVV VDSMREYLLE 
601:	KESSSVSSVF TVTGFNFAGR GQSSGMAFIM LKPWEERPGG ENSVFELAKR AQMHFFSFKD 
661:	AMVFAFAPPS VLELGNATGF DLFLQDQAGV GHEVLLQARN KFLMLAAQNP ALQRVRPNGM 
721:	SDEPQYKLEI DDEKASALGV SLADINSTVS IAWGSSYVND FIDRGRVKRV YLQGRPDARM 
781:	NPDDLSKWYV RNDKGEMVPF NAFATGKWEY GSPKLERYNG VPAMEILGEP APGLSSGDAM 
841:	AAVEEIVKQL PKGVGYSWTG LSYEERLSGS QAPALYALSL LVVFLCLAAL YESWSIPFSV 
901:	MLVVPLGVIG ALLATSMRGL SNDVFFQVGL LTTIGLSAKN AILIVEFAKE LHEQGKGIVE 
961:	AAIEACRMRL RPIVMTSLAF ILGVVPLAIS TGAGSGSQHA IGTGVIGGMV TATVLAIFWV 
1021:	PLFYVAVSTL FKDEASKQQA SVEKGQ