4.A.7.1.6
The PTS ascorbate transporter subunits IIBC (596 aas and 11 TMSs) and IIA (155 aas).The 3-d structure has been determined at high resolution of the inward open configuration, showing that ascorbate translocation can be achieved by a rigid-body movement of the substrate-binding core domain relative to the V motif domain, which brings along the transmembrane helices TM2 and TM7 of the V motif domain to undergo a winding at the pivotal positions (Luo et al. 2018). This completes the picture of the transport cycle of the ascorbate superfamily of membrane-spanning EIIC components of the PTS.
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| Accession Number: | PNQ00486 |
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| Protein Name: | PNQ00486.1 PTS ascorbate transporter subunit IIBC [Pasteurella multocida] |
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| Length: | 597 |
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| Molecular Weight: | |
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| Species: | Pasteurella multocida [747] |
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| Number of TMSs: | 11 |
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| Substrate |
L-ascorbate |
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1: METLYNLFLS FNNQVLSKAP FLLGIVACIG YILLKKDTTT VIKGTIKTIV GFMIVQAGSG
61: FLVANFKPII EGLSKYHNLT GAVIDPYTSM QATIQTMADN YAWVGYAVIL ALFLNILLVV
121: CRRITGIRTI MLTGHIMFQQ AGLVAVFYMI IGASMWETVI YTAVLMALYW GISSNIMYKP
181: TQAVTGGAGF SIGHQQQIAS WIAVKLAPKL GDKNDTVDKM KLPKWLHIFH DSISATALVM
241: TVFFGIILLS FGLDNLQQMA GKTHWFMYIF EMGLKFAVAI QIIVTGVRMF VAELSEAFKG
301: ISERVIPNSV LAIDCAAIYA FSPNAMVFGF MWGAIGQFVA VGLLLGFSSP ILIIPGFIPM
361: FFSNATIGVF ANQFGGWKSV MKICFIMGII EVLGSAWVIH LLATQGTAFN GWMGMADWAL
421: FFPPILQGIV SIPGFFFVLL TLAIVYMVFA SKQLRLEEAA AAALGKTLEQ MDGYDKTEHS
481: PITPDDSVQH DVLKTAVNPI RILAVCGSGQ GSSMMMKMKI KSYLDKHNIP NTLDSCAVTD
541: YKGKLNEIDI IVASRHLADE IEVGEDKFVL GVQNMLNPNS FGPELIALIK KYQQTT