3.A.20.1.3 Glycosomal membrane protein insertion apparatus. Two proteins included in the system are PEX14 and PEX19 which target to the peroxisome in several other organisms, but in T. brucei, they target to the glycosome. By contrast, PEX10 and PEX12 are involved in targetting to the peroxisome (Saveria et al. 2007). Altogether, PEX1, 2, 5, 6, 10, 11, 12, 14, 16, and 19 have been identified in T. brucei and are listed here. Inhibitors of PEX14 block protein import into glycosomes and kill Trypanosma parasites (Dawidowski et al. 2017). The glycosomal membrane-associated Leishmania donovani protein PEX14, which plays a crucial role in protein import from the cytosol to the glycosomal matrix, consists of three domains: an N-terminal domain where the signalling molecule binds, a transmembrane domain and an 84-residue coiled-coil domain (CC) that is responsible for oligomerization. CCs are versatile domains that participate in a variety of functions including supramolecular assembly, cellular signalling and transport (Shakya and Pratap 2020). Bioinformatic analyses indicate that the N-terminal region of TbPEX11 contains an amphiphilic helix and several putative TOM20 recognition motifs. Thus, the extreme N-terminal region of TbPEX11 contains a cryptic N-terminal signal that directs PEX11 to the mitochondrion if its glycosomal transport is blocked (Krishna et al. 2023).
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Accession Number: | Q38IE1 |
Protein Name: | PEX6 |
Length: | 982 |
Molecular Weight: | 106011.00 |
Species: | Trypanosoma brucei [5691] |
Number of TMSs: | 1 |
Substrate |
protein polypeptide chain |
---|
1: MMSRTVELER CILRTKTYEA YKNVLQQSTS MTDHAQSLVN TLIQTHGEMH LGEKIPSGDG
61: RPVEFVVVDL VPATVGTLTE STTVIVLPPS SDGKELPPTA AMGTTGSSAE GTGADGVSTN
121: NELERGGICS VRLDYSLSGS STAASEAFLL STLLQDGSIV CVGDEEYTTA FTLVRQRDDP
181: VPTTEQQEQA ETQRRKQQCD TLPTLYASPS VFRNIRAFLM LDVLPTIMKS RFQYNDDQLC
241: VPVSLFREVH NGSGAPITIE AVSSGSRKEL HLSAVAVTPA WPVFILTEVF GESYETLLTA
301: TLKASERALH GRIVAEGDVI VLSVTPTQLV EMQLEPNTAV EAVLVQVIHA VQSGCALPAV
361: VTSVLHDGAI AIPFRVLEVN TLSGAAYGRI ELEDKGNGTE LLLLLQIALG SPDTTPLFPP
421: PPTPPTFFPQ LSLTMNIRSA LERCYVPSNA GTTSVFVVHA TKENLPVECV SMVMALLGVE
481: CLLVDMERKS DEEVQRLLGA YMSCSGEVAL VIRNATKLDQ RTELLRLFDA DNSGNGVDGT
541: CSDGDCLNGI RVIFLVCECV EAPPPAVAAQ ARNVEGVLKG THPSEKDRRL IVENIFTGAQ
601: RSRGFCKSLL LSFDSVASWT VGLSTVDVVA YAEECVSVLA SMSLPDGVLP VLSESLCSSI
661: LEKFQKAHGH NLVSTKLQPV RWSDVGGLED AKRELREMIQ LPLLYPELLG NGGNAKHGAG
721: ILFYGPPGCG KTLLAKAVAT EMNMNFMAVK GPELINQYVG ESEKNIRLLF QRARDNSPCI
781: IFFDELDALA PARGAKGDAG GAMDRVVAQL LVEVDGVGHS RSDGTAAGKV FIIAATNRPD
841: LLDPALLRPG RFDKLCYLGI PSTRSEQLVA LRALTRKFHL AEDVDLEALL QPMTLDYTGA
901: DLFALCSDAM MFAVEAMLQE SLTFNEEGSP SMLAEPAKNL VVRMNDFVRA RDQLKPSVTA
961: EDLRRYESLR TKFTANSGRV AD