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3.A.7.9.1
The Icm/Dot or Dot/Icm multicomponent protein secretion system. IcmS and IcmW form a complex that interacts with and may translocate substrate proteins (Ninio et al., 2005; De Buck et al., 2007; Cambronne and Roy, 2007). The crystal structure of the IcmR-IcmQ complex has been solved (Raychaudhury et al., 2009). Legionella pneumophila survives and replicates inside host cells by secreting ~300 effectors through the defective in organelle trafficking (Dot)/intracellular multiplication (Icm) type IVB secretion system (T4BSS). Ghosal et al. 2019, using electron cryotomography mapped the location of the core and accessory components of the Legionella core transmembrane subcomplex, revealing a well-ordered central channel that opens into a large, windowed secretion chamber with an unusual 13-fold symmetry. Immunofluorescence microscopy deciphered an early-stage assembly process that begins with the targeting of Dot/Icm components to the bacterial poles. Polar targeting of this T4BSS is mediated by two Dot/Icm proteins, DotU and IcmF, that are homologues of the T6SS membrane complex components TssL and TssM, suggesting that the Dot/Icm T4BSS is a hybrid system. Thus, the Dot/Icm complex assembles in an 'axial-to-peripheral' pattern (Ghosal et al. 2019).

Accession Number:Q5ZS43
Protein Name:DotB
Length:387
Molecular Weight:43602.00
Species:Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513) [272624]
Location1 / Topology2 / Orientation3: Cell membrane1 / Single-pass membrane protein2
Substrate proteins

Cross database links:

RefSeq: YP_096681.1   
Entrez Gene ID: 3079449   
Pfam: PF00437   
BioCyc: LPNE272624:LPG2676-MONOMER   
KEGG: lpn:lpg2676   

Gene Ontology

GO:0005622 C:intracellular
GO:0005524 F:ATP binding
GO:0006810 P:transport

References (1)

[1] “The genomic sequence of the accidental pathogen Legionella pneumophila.”  Chien M.et.al.   15448271

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FASTA formatted sequence
1:	MATYYSTSKL MSMDNINLMP DEPTRFTPVF MDRMLEHAES LNASDITIQT GEPIFAEVYG 
61:	RLLKITNRRL SNTELGDLIN SIYGPNATTQ LLSGKDIDTH YEFRPNRGVR YRYRVNATAC 
121:	LVEGHDAIQI TLRTIPTTPP KLSTMNLPDN IIEAIAPQEG IVFITGATGS GKSTLLASII 
181:	RELIETSDSN RKVLTYESPI EFVYDEIETI SAVVSQSEIP RHLPNFADGV RNALRRKPRL 
241:	IMVGECRDAE TISAALEAAL TGHPVYTTLH TSGVAETMRR LVTSFSGEER LGRTIDILET 
301:	IRLCIWQKLV PTVDERRVAL REYLVFDEEV RDILLEGDPN EVTSATRKLV RQKGQLMTWD 
361:	AKMKFEQGII SERVYKLIIA GAKEYQQ