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3.A.7.9.1
The Icm/Dot or Dot/Icm multicomponent protein secretion system. IcmS and IcmW form a complex that interacts with and may translocate substrate proteins (Ninio et al., 2005; De Buck et al., 2007; Cambronne and Roy, 2007). The crystal structure of the IcmR-IcmQ complex has been solved (Raychaudhury et al., 2009). Legionella pneumophila survives and replicates inside host cells by secreting ~300 effectors through the defective in organelle trafficking (Dot)/intracellular multiplication (Icm) type IVB secretion system (T4BSS). Ghosal et al. 2019, using electron cryotomography mapped the location of the core and accessory components of the Legionella core transmembrane subcomplex, revealing a well-ordered central channel that opens into a large, windowed secretion chamber with an unusual 13-fold symmetry. Immunofluorescence microscopy deciphered an early-stage assembly process that begins with the targeting of Dot/Icm components to the bacterial poles. Polar targeting of this T4BSS is mediated by two Dot/Icm proteins, DotU and IcmF, that are homologues of the T6SS membrane complex components TssL and TssM, suggesting that the Dot/Icm T4BSS is a hybrid system. Thus, the Dot/Icm complex assembles in an 'axial-to-peripheral' pattern (Ghosal et al. 2019).

Accession Number:Q5ZYB4
Protein Name:IcmF
Length:973
Molecular Weight:110807.00
Species:Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513) [272624]
Location1 / Topology2 / Orientation3: Cell membrane1 / Single-pass membrane protein2
Substrate proteins

Cross database links:

RefSeq: YP_094502.1   
Entrez Gene ID: 3079541   
Pfam: PF06744    PF06761   
BioCyc: LPNE272624:LPG0458-MONOMER   
KEGG: lpn:lpg0458   

References (1)

[1] “The genomic sequence of the accidental pathogen Legionella pneumophila.”  Chien M.et.al.   15448271

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FASTA formatted sequence
1:	MDNSLRALCD AIKKILSQLK PQTNQLSFVV ITGKNAQGKS ALLKQSNMEE MPVFSEEHAK 
61:	LYFNQKGIIV ELGENWLTNS KTLLLTTLKQ LNKCNRHLKI TGLILCVDVN DLLIAEPAQF 
121:	TEQKKSHLQL IQRLGVNLGY QAELALIFTK MDTLAGFSEF YQMDHVNDLS KPLGFSLDCV 
181:	NQLSKKIETF SLQFNQLIEA LGQQVINKMH PARSTIKRSL IREFPLQLAS LRAPIQSLIQ 
241:	GISPKLFHVH SIFFTSAEQG GVSVDRLNKK IQHEYALVVQ DTFPQATNFR SYFVEGALQT 
301:	IQEQCIQVPQ TTKFSQKPFI AVAVSLAGLS LLTLVYNHYK TSHILDEASK ELLAYEALNN 
361:	QGTSGAQALY HLSQAANKMN HITSNSISLP TVHQLKLNLH NNAQNRLQGE FLPSLANELE 
421:	QVITSPGNTP ITRYKALKIY LMLGNSKYFS SQPILDWFQQ QWQGRAPGTT QKQLALLKQT 
481:	LSNPPQNIPI KSQIISDARN YLNALPTSYL YYSIAKEYFP QEKQKIVIEG FSIPTDEVPT 
541:	YFTKTGFTKI INELPKITST LQSENWVLAR QDLSQLHNML IQAYCFDYVT WWQTFMKKSQ 
601:	PLRYQDYQQG RQLTKLIQQS NSFYKFANLI QQETKPDLTE GISTFNQFIA NQFTDLNLTT 
661:	LSSIKELDFR ISELERFIAT LSVVNDGGKT AFTITRSRFL NENSSDPVSL LYSQARQLPE 
721:	PLSSWTKQIA GDIWVILIRD SRQYINNQWQ KTVYREFQNS IAQRYPFDSS QTAEIDIADF 
781:	NRFFSTHGLL NTYSEQYIKP FLDTSSPEWK PKAVNDFILP ISNEVIDELI RANIITNMFF 
841:	PDHSDESKID FSLQKISLDP VVSNLVLEIG GTKLMDNQTS DSLIRFTWPQ NNAKLALDSI 
901:	EGNHYELAEY GTWAIFKLLE KVNVLVDEQD SSSLQILFEV NSNSGRYLLK TNNQINPFTP 
961:	GILNGFTLND HIA