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3.A.7.9.1
The Icm/Dot or Dot/Icm multicomponent protein secretion system. IcmS and IcmW form a complex that interacts with and may translocate substrate proteins (Ninio et al., 2005; De Buck et al., 2007; Cambronne and Roy, 2007). The crystal structure of the IcmR-IcmQ complex has been solved (Raychaudhury et al., 2009). Legionella pneumophila survives and replicates inside host cells by secreting ~300 effectors through the defective in organelle trafficking (Dot)/intracellular multiplication (Icm) type IVB secretion system (T4BSS). Ghosal et al. 2019, using electron cryotomography mapped the location of the core and accessory components of the Legionella core transmembrane subcomplex, revealing a well-ordered central channel that opens into a large, windowed secretion chamber with an unusual 13-fold symmetry. Immunofluorescence microscopy deciphered an early-stage assembly process that begins with the targeting of Dot/Icm components to the bacterial poles. Polar targeting of this T4BSS is mediated by two Dot/Icm proteins, DotU and IcmF, that are homologues of the T6SS membrane complex components TssL and TssM, suggesting that the Dot/Icm T4BSS is a hybrid system. Thus, the Dot/Icm complex assembles in an 'axial-to-peripheral' pattern (Ghosal et al. 2019).

Accession Number:Q5ZYB5
Protein Name:TphA aka ProP
Length:418
Molecular Weight:46069.00
Species:Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513) [272624]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell membrane1 / Single-pass membrane protein2
Substrate proteins

Cross database links:

RefSeq: YP_094501.1   
Entrez Gene ID: 3080272   
Pfam: PF07690   
BioCyc: LPNE272624:LPG0457-MONOMER   
KEGG: lpn:lpg0457   

Gene Ontology

GO:0016020 C:membrane
GO:0005215 F:transporter activity
GO:0055085 P:transmembrane transport

References (1)

[1] “The genomic sequence of the accidental pathogen Legionella pneumophila.”  Chien M.et.al.   15448271

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MKQNTKHSII AGLYGNALEW YDFLLYASFA PVFAEIFFPS KIHFVSLIAT FSLFAIGFIM 
61:	RPIGGLLLGH YADHVGRRKA LILSMSVMTI STALIAVLPD FNSWGIIAPL LFSVLRLIQG 
121:	LAVGGELPGS TTYLIEHMFQ QSRGLAGSLV LSSAFLGIFI GALTASIFST LFTGDALLNW 
181:	GWRYAYLLGG ILGILGIYLR IKSFESPTFL KDKHTAELPA KVVFTRFKQQ LFLSVIITSI 
241:	LAMSNYVLIA YVTTFLVKSE YFLLKDALLI NLIALALLTV LIPLMGFLSD VVGRKPIFLI 
301:	GLFALCLLIF PFFWLLLSGS WWKALCSELL LAIALAPINA TVPTIIAEMF PTSVRASGIS 
361:	ISYNIGQALF GGTMPLVAFS LIELSSNKMA PAWYLFLWTL IVIPITISIR ESYQKELT