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3.A.7.9.1
The Icm/Dot or Dot/Icm multicomponent protein secretion system. IcmS and IcmW form a complex that interacts with and may translocate substrate proteins (Ninio et al., 2005; De Buck et al., 2007; Cambronne and Roy, 2007). The crystal structure of the IcmR-IcmQ complex has been solved (Raychaudhury et al., 2009). Legionella pneumophila survives and replicates inside host cells by secreting ~300 effectors through the defective in organelle trafficking (Dot)/intracellular multiplication (Icm) type IVB secretion system (T4BSS). Ghosal et al. 2019, using electron cryotomography mapped the location of the core and accessory components of the Legionella core transmembrane subcomplex, revealing a well-ordered central channel that opens into a large, windowed secretion chamber with an unusual 13-fold symmetry. Immunofluorescence microscopy deciphered an early-stage assembly process that begins with the targeting of Dot/Icm components to the bacterial poles. Polar targeting of this T4BSS is mediated by two Dot/Icm proteins, DotU and IcmF, that are homologues of the T6SS membrane complex components TssL and TssM, suggesting that the Dot/Icm T4BSS is a hybrid system. Thus, the Dot/Icm complex assembles in an 'axial-to-peripheral' pattern (Ghosal et al. 2019).

Accession Number:Q5ZYC1
Protein Name:DotG aka IcmE
Length:1048
Molecular Weight:107807.00
Species:Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513) [272624]
Number of TMSs:2
Location1 / Topology2 / Orientation3: Cell membrane1 / Single-pass membrane protein2
Substrate protein polypeptide chain

Cross database links:

RefSeq: YP_094495.1   
Entrez Gene ID: 3078914   
Pfam: PF03743   
BioCyc: LPNE272624:LPG0451-MONOMER   
KEGG: lpn:lpg0451   

References (1)

[1] “The genomic sequence of the accidental pathogen Legionella pneumophila.”  Chien M.et.al.   15448271

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FASTA formatted sequence
1:	MASKKENLKS LFSNTRTRVI IIFTAALLII AVVIGFFKIR GATTGSIAAA EVSTVPGGIQ 
61:	SIPGVLDPTA QYAKLQEEQN ITQAQVAEKT GGSAIPTIIR TQALGEGVGV IGSQSGVGFA 
121:	ALAQEELGGP QRSLWIQELQ DGSCSKSVIT KVVNQGAQLT DLKAACSCVQ LKDSGYGLQE 
181:	LEQVCECKEL KSAGYNARQL KEAGYSAGRL RNCGFDACEL RNAGFTAQEM KDGGFSDGEL 
241:	KGAGFSDAEI AKASGLPDGI TADDVRKAGC GAAALAKLRQ AGVSASAIRK ISGCTAEQLK 
301:	AAGYTAKELK DAGFSAADLR RAGFSAAELK DAGFTARDLL NAGFTPADLA KAGFSDAQIK 
361:	AAQAELPPGI TPQDVKNAGC DVEALKKERE AGVSAALIRQ YAGCSAQALK AAGFTDADLA 
421:	NAGFTPAQIS AATPLSDAEI KAAGCDPDKL KKLFSAGVSA KRIKELNGCS AEALKAAGYD 
481:	AQSLLAAGFT PQELLAAGFT PKQLEDAGLN PVSIIADGRV ADCSVESLKK ARAAGVSALT 
541:	IKQTLGCSAA ALKAAGYTAK ELKDAGFTAA ELKAAGFSAK ELKDAGFTAK ELRDAGFSAQ 
601:	ELKDVGFSAK DLKDAGFSAA ELKAAGFTAA QLKAAGFSAK DLKDAGFSAA ELKAAGFSAK 
661:	ELKDAGFSAS DLKNAGFSAK ELKDAGFSAS DLKSAGFSAS ELKNAGYSAD ELKKAGYTSA 
721:	ELRNAGFSPQ ESAVAGLQGP DLQQLDSSIT GIPSIPGATP RPTTSDAASS AEQLQAILQK 
781:	QNEQLAEQKY QQEIQQRTSD MLTAATQLVQ DWKQVETQVY TEGTEETKTS GGESAVPGTG 
841:	TGTGSNNQPV DQGAVSAQNQ AIIKTGDIMF AVLDTSVNSD EPGPILATIV TGKLKGSKLI 
901:	GSFNLPSNAD KMVITFNTMS IPGAEKTISI SAYAIDPNTA RTALASRTNH HYLMRYGSLF 
961:	ASSFLQGFGN AFQSANTTIT IGGTGGGNNI TVANGVGRST LENAVIGLAT VGKAWSQQAQ 
1021:	QLFNTPTTVE VYSGTGLGIL FTQDVTTI