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3.A.7.9.1
The Icm/Dot or Dot/Icm multicomponent protein secretion system. IcmS and IcmW form a complex that interacts with and may translocate substrate proteins (Ninio et al., 2005; De Buck et al., 2007; Cambronne and Roy, 2007). The crystal structure of the IcmR-IcmQ complex has been solved (Raychaudhury et al., 2009). Legionella pneumophila survives and replicates inside host cells by secreting ~300 effectors through the defective in organelle trafficking (Dot)/intracellular multiplication (Icm) type IVB secretion system (T4BSS). Ghosal et al. 2019, using electron cryotomography mapped the location of the core and accessory components of the Legionella core transmembrane subcomplex, revealing a well-ordered central channel that opens into a large, windowed secretion chamber with an unusual 13-fold symmetry. Immunofluorescence microscopy deciphered an early-stage assembly process that begins with the targeting of Dot/Icm components to the bacterial poles. Polar targeting of this T4BSS is mediated by two Dot/Icm proteins, DotU and IcmF, that are homologues of the T6SS membrane complex components TssL and TssM, suggesting that the Dot/Icm T4BSS is a hybrid system. Thus, the Dot/Icm complex assembles in an 'axial-to-peripheral' pattern (Ghosal et al. 2019).

Accession Number:Q5ZYC7
Protein Name:IcmP
Length:380
Molecular Weight:43814.00
Species:Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513) [272624]
Number of TMSs:3
Location1 / Topology2 / Orientation3: Cell membrane1 / Single-pass membrane protein2
Substrate proteins

Cross database links:

RefSeq: YP_094489.1   
Entrez Gene ID: 3077691   
BioCyc: LPNE272624:LPG0445-MONOMER   
KEGG: lpn:lpg0445   

References (1)

[1] “The genomic sequence of the accidental pathogen Legionella pneumophila.”  Chien M.et.al.   15448271
Structure:
6EXA   6EXB   6EXC   6EXD   6EXE   6SZ9     

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MYIEMAQQQQ QSGSDNSMAP VWIVILLFIT AYFVWALAHQ YIVSFVFTIN IWQARLVNLF 
61:	LNNQLLANQI YLMQTLDPNT VNWDQMVTVM RAVGDYMRYP VICILVVLAF VLYNSNVTLK 
121:	YRKTYDMKSL RAQEQFNWPA IMPIVKEDLV SQDVNKGPWA MALTPMEFAR KYNLLRKDDA 
181:	LLDNPVPGEE MTAGIRRGDA KRVFTMQLGP YWDGFERCSP QAYALSAVFM ARMNRDRDAA 
241:	NNILKVLDKT FVDGKPDFSV ARPVMKKYQN SELVQEVVAK HAYVLTVIAS LLEAAREDGV 
301:	VPSSEFLWLK PVDRRLWYML NCVGRQTPYS EVAGPFAHWK AEKEMGRRSL VPMIDEAIRA 
361:	LEIAVKEVRL TPRQMEELEP