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3.A.1.135.5
The hetrodimeric ABC transporter, TM287/TM288.  The 2.9-Å crystal structure has been solved in the inward-facing state. The two nucleotide binding domains (NBDs) remain in contact through an interface involving conserved motifs that connect the two ATP hydrolysis sites.  AMP-PNP binds to a degenerate catalytic site which deviates from the consensus sequence in the same positions as the eukaryotic homologs, CFTR (TC# 3.A.1.202.1) and TAP1-TAP2 (TC# 3.A.1.209.1) (Hohl et al. 2012).  The structural basis for allosteric crosstalk (positive cooperativity) between the two ATP binding sites has been studied (Hohl et al. 2014).  The two NBDs exhibit unexpected differences and flexibility (Bukowska et al. 2015). It exports daunomycin and the nonfluorescent 2,7-bis(carboxyethyl)-5(6)-carboxyfluorescein-acetoxymethylester (BCECF-AM) (Hohl et al. 2012). Timachi et al. 2017 observed hydrolysis-independent closure of the NBD dimer, further stabilized as the consensus site nucleotide is committed to hydrolysis.

Accession Number:Q9WYC4
Protein Name:Uncharacterized ABC transporter ATP-binding protein TM_0288
Length:598
Molecular Weight:67708.00
Species:Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099) [243274]
Number of TMSs:5
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate Daunomycin, 2,7-bis(carboxyethyl)-5(6)-carboxyfluorescein-acetoxymethylester

Cross database links:

Structure:
3QF4   4Q4A   4Q4H   4Q4J   4Q7L   4Q7M   6QUZ   6QV0   6QV1   6QV2   [...more]

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MPEIRRRPHG PILEKPALKN PTATLRRLLG YLRPHTFTLI MVFVFVTVSS ILGVLSPYLI 
61:	GKTIDVVFVP RRFDLLPRYM LILGTIYALT SLLFWLQGKI MLTLSQDVVF RLRKELFEKL 
121:	QRVPVGFFDR TPHGDIISRV INDVDNINNV LGNSIIQFFS GIVTLAGAVI MMFRVNVILS 
181:	LVTLSIVPLT VLITQIVSSQ TRKYFYENQR VLGQLNGIIE EDISGLTVIK LFTREEKEME 
241:	KFDRVNESLR KVGTKAQIFS GVLPPLMNMV NNLGFALISG FGGWLALKDI ITVGTIATFI 
301:	GYSRQFTRPL NELSNQFNMI QMALASAERI FEILDLEEEK DDPDAVELRE VRGEIEFKNV 
361:	WFSYDKKKPV LKDITFHIKP GQKVALVGPT GSGKTTIVNL LMRFYDVDRG QILVDGIDIR 
421:	KIKRSSLRSS IGIVLQDTIL FSTTVKENLK YGNPGATDEE IKEAAKLTHS DHFIKHLPEG 
481:	YETVLTDNGE DLSQGQRQLL AITRAFLANP KILILDEATS NVDTKTEKSI QAAMWKLMEG 
541:	KTSIIIAHRL NTIKNADLII VLRDGEIVEM GKHDELIQKR GFYYELFTSQ YGLVVEKE