3.A.1.25.5 Biotin/Riboflavin ECF transport system, EcfAA'T/RibU/BioY (Karpowich and Wang 2013). RibU binds riboflavin with high affinity, and the protein-substrate complex is
exceptionally stable in solution. The crystal structure of riboflavin-bound RibU reveals an
electronegative binding pocket at the extracellular surface in which the substrate is completely
buried (Karpowich et al. 2016).
|
Accession Number: | Q9X1Z1 |
Protein Name: | Energy-coupling factor transporter ATP-binding protein EcfA |
Length: | 259 |
Molecular Weight: | 29376.00 |
Species: | Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099) [243274] |
Location1 / Topology2 / Orientation3: |
Cell inner membrane1 / Peripheral membrane protein2 |
Substrate |
biotin, riboflavin |
---|
1: MKITLNSVSF RYNGDYVLKD VNAEFETGKI YVVVGKNGSG KTTLLKILAG LLEAEGEIFL
61: DGSPADPFLL RKNVGYVFQN PSSQIIGATV EEDVAFSLEI MGLDESEMRK RIKKVLELVG
121: LSGLEKEDPL NLSGGQKQRL AIASMLARDT RFLALDEPVS MLDPPSQREI FQVLESLKNE
181: GKGIILVTHE LEYLDDMDFI LHISNGTIDF CGSWEEFVER EFDDVEIPFK WKLWKKCGKI
241: NLWEDRYENS GNQRRRDTV