1.A.3.1.9
Ryanodine-sensitive Ca2+ release channel RyR1 of 5117 aas and 6 TMSs. Diamide insecticides, such as flubendiamide and chlorantraniliprole, selectively activate insect ryanodine receptors of Lepidoptera and Coleoptera pests (Samurkas et al. 2020). They are particularly active against lepidopteran pests of cruciferous vegetable crops, including the diamondback moth, Plutella xylostella. Resistance results from mutation(s) in the ryanodine receptors' transmembrane domain at the C-termini of these proteins (Troczka et al. 2017). Other diamide insecticides, including phthalic and anthranilic diamides, target insect ryanodine receptors (RyRs) and cause misregulation of calcium signaling in insect muscles and neurons. Homology modeling and docking studies with the diamondback moth ryanodine receptor revealed the mechanisms for channel activation, insecticide binding, and resistance (Lin et al. 2019).
|
| Accession Number: | G8EME3 |
|---|
| Protein Name: | Ryanodine receptor 1 |
|---|
| Length: | 5117 |
|---|
| Molecular Weight: | 578482.00 |
|---|
| Species: | Plutella xylostella (Diamondback moth) [51655] |
|---|
| Number of TMSs: | 6 |
|---|
| Substrate |
cation, calcium ion |
|---|
1: MAEAEGGASE QDDVSFLRTE DMVCLSCTAT GERVCLAAEG FGNRHCFLEN IQDKNIPPDL
61: SQCVFVIEQA LSVRALQELV TAAGSETGKG TGSGHRTLLY GNAILLRHLN SDMYLACLST
121: SSSQDKLAFD VGLQEHSQGE ACWWTLHPAS KQRSEGEKVR VGDDLILVSV ATERYLHTTK
181: ENEVSIVNAS FHVTHWSVQP YGTGISRMKY VGYVFGGDVL RFFHGGDECL TIPSSWSNEG
241: QHNIVVYEGG SVMSQARSLW RLELARTKWA GGFINWYHPM RIRHITTGRY LAVNDQNELY
301: LVSREEATTA SCAFCLRQEK DDQKVVLEDK DLEIIGAPII KYGDSTVIVQ HSESSLWLSY
361: KSYETKKKGL GKVEEKQAIL HEEGKMDGLD FSRSQEEESR TARVIRKCSS LFTKFINGLE
421: TLQENRRHSM FFATVNLAEM VMCLEDLINY FAQPDEDMEH EEKQNKFRAL RNRQDLFQEE
481: GILNLILEAI DKINVITSQG FLAGFLAGDE CGQSWEMISA YLYQLLAAII KGNHTNCAQF
541: ANSNRLNWLF SRLGSQASGE GTGMLDVLHC VLIDSPEALN MMRDEHIKVI ISLLEKHGRD
601: PKVLDVLCSL CVGNGVAVRS SQNNICDYLL PGKNLLLQTQ LVDHVSSVRP NIFVGRVEGS
661: AVYQKWYFEV TMDHIEKTTH MMPHLRIGWA NTTGYVPYPG GGEKWGGNGV GDDLYSYGYD
721: GAFLWSGGRK TGVNRTHAEE PYIRKGDVIG CALDLTVPII NFMFNGVRVT GSFTNFNLEG
781: MFFPVISCSS KLSCRFLLGG EHGRLRYAAP EGYSPLVECL LPQQILSLEP CFCFGNLAKR
841: ALSGPPLVQD DTAFVPMPVD TLTITLPSYI EQIRDKLAEN IHEMWAMNKI EAGWMYGEHR
901: ENLHKIHPCL VPFERLPAAE KRYDIQLAVQ TLKTILALGY YISLDKPPAR IRNVRLPNEP
961: FLQSNGYKPA PLDLSAVTLT PKMDELVEQL AENTHNLWAR ERIQQGWTYG LNEDSDMHRS
1021: PHLVPYPKVD EAIKKANRDT ASETVRTLLV YGYMLDPPTG EHEALLAEAS KMKQADFRTY
1081: RAEKNYAVNS GKWYFEFEIL TAGPMRVGWA HADMPPGMML GQDENSWAFD GYNEEKVYVN
1141: SSESFGKQWA VGDVVGVFLD LIDNTISFSL NGELLMDALG GETTFADVQG DNFVPACTLG
1201: VGRKARLTYG QDVNTLKYFT TCGLQEGYEP FCVNMKRDVT HWYTKDQPIF ENTDEMADTR
1261: IDVTRIPAGS ETPPCMKISH NTFETMEKAN WEFLRLSLPV ICHAEFIDEN EKARRWVEIK
1321: ERQQALMKEA AEAQMPAHID QIMRSGFTMN DIKGLHRDYD DGQEEPSNSK MKRLPSRPPR
1381: KGSLSRGVTI QNYSLQPGQQ NGMHRTASEM EMSKYESGAQ SVTDDKKDKR GRSPFKFFSR
1441: SKRGESGDRK SRKSKTPDPF SDAEVSPERA APRRPNPQIR VSQANQRYEK NQSRQNLYGS
1501: QGGGLNNGNT NLQVNTPPVE RKQMTGGALS GATTETVGNE IFDAECLKLI NEYFYGVRIF
1561: PGQDPTHVYI GWVTTQYHLH SKDFNQSKVT KSSVIITDDY DRVIRSVNRQ SCYMVRADEL
1621: YNEVMAEAVG KGASQGMFVG CSVDTSTGTV AFTCEGKETS FRFSMEPETK LFPAIFVEAT
1681: SKEILQIELG RSLHSLPLSA AVLPTSDKHV IPQFPPRLKV QCLKPHQWAR VPNQSLQVHA
1741: LKLSDIRGWS MLCEDAVSML ALHIPEEDRC IDILELIEMD KLLSFHSHTL TLYAALCYQS
1801: NYRAAHALCQ HVDQKQLLYA IQSQYMSGPL RQGFYDLLIA LHLESHATTM EVCKNEYVIP
1861: LGPELKALYE EPEMGHSLRS LQTESVRPMM KMTDIAESIN EISNLYSPYF PLEVVREFVM
1921: QALAEAVETN QVHNRDPVGG SNENLFLPLI KLVDRLLLVG MMRDEDVEKL LIMTNPETWD
1981: PTFDKEGKDE HKKGLLHMKM AEGAKLQMCY LLHHLNDIQL RHRVEAIIAF AHDFVGDLQA
2041: DQLRRYVEIK QSDLPSAVAA KKTREYRCPP REQMNAILSF KHLEEDDKEN IPCGEDLIAR
2101: MNDFHQALMA HVSLMALQEP EVEENPEALN KPGAIGKLYN IINTVKELDE EPKAIEEPVK
2161: KTPEEKFRKV LIQTIVSWAE ESQIETPKLV REMFSLLVRQ YDAVGELVRA LEKTYVINAK
2221: TKLDVAEMWV GLSQIRALLP VQMSQEEEEL MRKRLWKLVN NHTFFQHPDL IRVLRVHENV
2281: MAVMMNTLGR RAQAQSDATP NQPAAEEKDT SHEMVVACCR FPCYFCRTGR QNQKAMFDHF
2341: DFLLENSNIL LSRPSLRGST PLDVAYSSLM ENTELALALR EHYLEKIAVY LSRCGLQSNS
2401: ELVEKGYPDL GWDPVEGERY LDFLRFCVWV NGESVEENAN LVIRLLIRRP ECLGPALRGE
2461: GEGLLKAIVD ANKMSEKIAD RRKLHEEVGQ EDVAFSHPLP ESDEDEDYID TGAAILNFYC
2521: TLVDLLGRCA PDASVIALGK NESLRARAIL RSLVPLEDLQ GVLSLRFTLN NPAMGEERPK
2581: SDMPSGLIPG HKQSIVLFLE RVYGIETQEL FYRLLEEAFL PDLRAATMLD RNDGCESDMA
2641: LSMNRYIGNS ILPLLIKHAN FYNEAENYGS LLDATLHTVY RLSKNRMLTK GQREAVSDFL
2701: VALTSAMQPS HVAETAEETH GDVSKLSEYT TVALRLLTLH YERCAKYYGS TGGQGVYGAS
2761: SDEEKRLTMM LFSNIFDSLS KMDYEPELFG KALPCLIAIG CALPPDYSLS KNYDDEFYGK
2821: EQQTTGGPDN PQYNPQPINT SSVALNNDLN TIVQKFSEHY HDAWASRKIE NSWVYGENWS
2881: DSQKAHPRLK PYNMLNDYEK ERYKEPVRES LKALLAIGWS VEHSEVDIPS TNRSSMRRQS
2941: KSGGRPVDVV TDSATPFNYN PHPVDMTNLT LSREMQNMAE RLAENAHDIW AKKKKEELTV
3001: NGGGIHPQLV PYDLLTDKEK KKDRERSQEF LKYLQYQGYK LHRPSKATQS ETEQTATGVA
3061: IELRFAYSLL EKLIQYIDRA TINMKMLKPS TTFSRRSSFK TSTRDIKFFS KVVLPLMEKY
3121: FSTHRNYFIA VATATNNVGA ASLKEKEMVA ALFCKLASLL RSRLTAFGPD VRITVRCLQV
3181: LVKGIDAKSL VKNCPEFIRT SMLTFFNNVA DDLGHTIHYL QDGKYSHLRG THLKTSTSLG
3241: YINGVIMPVL TAMFDHLANC EYGADLLLDE IQVASYKMLG SLFTLGTDST LTHDRKYLKT
3301: EIERHKNALG SCLGAFSSTF PVAFLKPHLN KHNQFSLLNR IADHSLEAQD VMARMEQSMP
3361: TLETILGLVE QFVDSDKTYS DSPHVIDVVL PLLCSYLPFW WAQGPDNVTP SGGNHVTMVT
3421: ADHMNQLLKN VLKLIKKNIG NENAPWMTRI ATYAQQIIIN SSEELLRESF LPLAERVRKR
3481: TDNMFHKEES LRGFIKSSTD DTSQVESQIQ EDWQLLVRDI YSFYPLLIKY VDLQRNHWLR
3541: NNVNEAEELY NHVAEIFNIW SKSQYFFKEE QNFISANEID NMVLIMPTAT RRVTTVVDGA
3601: PQGGGKVKKK KKHRDKKRDK DKEVQASLMV ACLKRLLPVG LNLFAGREQE LVQHCKDRFL
3661: KKMTEQDVAE FAKTQLTLPD KIDPADEMSW QHYLYSKLGS KSKTTTAMET AAENKAKIID
3721: DTVERIVAMS KVLFGLHMID HPQQMSKNVY RSVVSIQRKR AVIACFRHLP LHQLPRHRAC
3781: NIFSRTYYEL WLEEENIGQE VMIEDLTQSF EDAELKKSDV EAEGEGKPDP LTQLVTTFCR
3841: GAMTERSGAL QEDPLCMSYA HIIAKSCGEE EEEGGEEEEE GAGEAEGEEE GGASIHEQEM
3901: EKQKLLFHQA RLADRGVAEM VLLHISASKG VPSDMVMKTL QLGISILRGG NIDIQMGMLN
3961: HLKDKKDVGF FTSIAGLMNS CSVLDLDAFE RNTKAEGLGV GLEGAAGEKN MHDAEFTCAL
4021: FRFIQLTCEG HNLEWQNYLR TQAGNTTTVN VVICTVDYLL RLQESIMDFY WHYSSKELID
4081: PAGKANFFKA IGVASQVFNT LTEVIQGPCT QNQQALAHSR LWDAVGGFLF LFSHMQDKLS
4141: KHSSQVDLLK ELLNLQKDMI TMMLSMLEGN VVNGTIGKQM VDTLVESASN VELILKYFDM
4201: FLKLKDLTTS ASFAEIDANN DGWVLPKDFK EKMEQQKSYT PEEIEFLLAC CETNHDGKLD
4261: YIGFCDRFHE PAKEIGFNLA VLLTNLSEHM PNEPRLARFL ETAGSVLNYF EPFLGRIEIM
4321: GGSKRIERVY FEIKESNIEQ WEKPQIKESK RAFFYSIVTE GGDKEKLEAF VNFCEDAIFE
4381: MTHASGLMAD AEEAGGGPKN REAAYMYMGD DDDDSRGKDP FRRGLQSVKD GIATAFSSLS
4441: PANMKQKIAD MSQMPPQELA VGFFKMFFYL FYYIGYGALV VVRYIFGVLL GLMRGPQVEE
4501: PPPEPTEEEK IGPSRHLPAL SATDDQGQMA VSAFGLDITK EDNGQVQIKP HESPGTSSPS
4561: SGEEAETAED GEHSGDEPQP PSLIDLLGGE QAKKQAQERI EAQAAQQAAM SAIEAESKKA
4621: VQAPAPSALS QVDLSQYTRR AVSFLARKFY TLKYVALVLA FCINFVLLFY KVSTLDAEPG
4681: EGSGIGDIIS GSGSGSGSGS GDGGSGEEDE DPIELVHIDE DYFYMEHVIK IAALLHSIVS
4741: LAILIGYYHL KVPLAIFKRE KEIARKLEFD GLYIAEQPED DDLKSHWDKL VISAKSFPVN
4801: YWDKFVKKKV RAKYSETYDF DSISNMLGME KTAFATQEDE GRGFFHYILS IDWRYQVWKA
4861: GVTITDNSFL YSLWYFSFSV MGNFNHFFFA AHLLDVAVGF KTLRTILQSV THNGKQLVLT
4921: VMLLTIIVYI YTVIAFNFFR KFYVQEEDDD INRNCHDMLT CFVFNLYKGV RAGGGIGDEL
4981: EPPDGDDSEA WRIVFDITFF FFIIVILLAI LQGLIIDAFG ELRDQLESVK EDMESNCFIC
5041: GINKDYFDKV PHGFDTHVQR EHNLANYMFF LMHLINKPDT EYTGQETYVW NMYTQRCWDF
5101: FPVGDCFRKQ YEDAMGE