1.B.96.1.1
OEP40 porin of 358 aas and 10 transmembrane β-strands. The
reconstituted recombinant OEP40 protein forms a high conductance
β-barrel ion channel with subconductant states in planar lipid bilayers. It is slightly cation-selective PK+/PCl- ≈ 4:1 and
rectifying (i⃗/i⃖ ≅ 2) with a slope conductance of Ḡmax ≅ 690
picosiemens. It has a restriction zone diameter of ≅ 1.4
nm and is permeable to glucose, glucose 1-phosphate and glucose
6-phosphate, but not for maltose. Moreover, channel properties are
regulated by trehalose 6-phosphate, which cannot permeate. Thus, OEP40 is a "glucose-gate" in the outer
envelope membrane of chloroplasts, facilitating selective metabolite
exchange between chloroplasts and the surrounding cytoplasm of the cell (Harsman et al. 2016).These porins and many others in eukaryotes have been reviewed and analyzed by Roumia et al. 2020.
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| Accession Number: | CAA0387124.1 |
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| Protein Name: | CAA0387124.1 unnamed protein product [Arabidopsis thaliana] |
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| Length: | 359 |
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| Molecular Weight: | |
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| Species: | Arabidopsis thaliana [3702] |
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| Substrate |
ion, anion, cation, D-glucopyranose, D-glucopyranose 1-phosphate, beta-D-glucose 6-phosphate |
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1: MKASMKFREE QKPLFRAKVP LSILGLPFQS GIVAGESKEL SLNLSTFFES GPSLKVAYRP
61: NDSWNPFSLI VKTGSGSFGS PISSSMLMSA EFNLLGQGNP SFMLHFKPQF GDFSIKKSHS
121: SSGFERNLIK SMNGSVSEDD SSIEVVDTPA VNGCGGGFRK VTVLPSTSAG DIAGLLSGVE
181: VAARTSLPVR GRAVLNFRWG VRVPTEIRRD FDPTAAISLR RFPFLVMNKI GIEHVDGADA
241: KVTKSTGDPG KVSGPAQFTT SGDVAEVIEE LRTENKQLKR AVEDLREVIS NVRPYSPATI
301: DYGSHSKYRE SERNNNNGRS RADRWSSERT TTSDYGGKKS KEEGNVAEEL KKALKGAA