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1.C.39.14.1
Macrophage-expressed gene 1 protein, Mpeg1, or perforin-2, PFN2, of 716 aas and one C-terminal TMS.  Pore formation has been demonstrated in target bacteria (McCormack et al. 2013). PFN2 undergoes a pre-pore to pore transition upon acidification (Jiao et al. 2021). Rawat and Jakubzick 2023 showed that channeling of antigens to CD8+ T cells is facilitated by perforin-2. It translcates the antigens to the cytosol in cross-presenting dendritic cells. A hexadecameric perforin-2 pore forms in phagosome membranes to fåcilitat transport of antigens to the cytosol (Rawat and Jakubzick 2023).  Cytosolic antigens use classical MHC-I pathway molecules for cross presentation (ubiquitination, proteasome degradation, and TAP transport into the endoplasmic reticulum).

Accession Number:Q2M385
Protein Name:Macrophage-expressed gene 1 protein
Length:716
Molecular Weight:78587.00
Species:Homo sapiens (Human) [9606]
Number of TMSs:1
Location1 / Topology2 / Orientation3: Membrane1 / Single-pass type I membrane protein2
Substrate molecule

Cross database links:

Structure:
6U23   6U2J   6U2K   6U2L   6U2W     

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MNNFRATILF WAAAAWAKSG KPSGEMDEVG VQKCKNALKL PVLEVLPGGG WDNLRNVDMG 
61:	RVMELTYSNC RTTEDGQYII PDEIFTIPQK QSNLEMNSEI LESWANYQSS TSYSINTELS 
121:	LFSKVNGKFS TEFQRMKTLQ VKDQAITTRV QVRNLVYTVK INPTLELSSG FRKELLDISD 
181:	RLENNQTRMA TYLAELLVLN YGTHVTTSVD AGAALIQEDH LRASFLQDSQ SSRSAVTASA 
241:	GLAFQNTVNF KFEENYTSQN VLTKSYLSNR TNSRVQSIGG VPFYPGITLQ AWQQGITNHL 
301:	VAIDRSGLPL HFFINPNMLP DLPGPLVKKV SKTVETAVKR YYTFNTYPGC TDLNSPNFNF 
361:	QANTDDGSCE GKMTNFSFGG VYQECTQLSG NRDVLLCQKL EQKNPLTGDF SCPSGYSPVH 
421:	LLSQIHEEGY NHLECHRKCT LLVFCKTVCE DVFQVAKAEF RAFWCVASSQ VPENSGLLFG 
481:	GLFSSKSINP MTNAQSCPAG YFPLRLFENL KVCVSQDYEL GSRFAVPFGG FFSCTVGNPL 
541:	VDPAISRDLG APSLKKCPGG FSQHPALISD GCQVSYCVKS GLFTGGSLPP ARLPPFTRPP 
601:	LMSQAATNTV IVTNSENARS WIKDSQTHQW RLGEPIELRR AMNVIHGDGG GLSGGAAAGV 
661:	TVGVTTILAV VITLAIYGTR KFKKKAYQAI EERQSLVPGT AATGDTTYQE QGQSPA