1.C.39.14.1
Macrophage-expressed gene 1 protein, Mpeg1, or perforin-2, PFN2, of 716 aas and one C-terminal TMS. Pore formation has been demonstrated in target bacteria (McCormack et al. 2013). PFN2 undergoes a pre-pore to pore transition upon acidification (Jiao et al. 2021). Rawat and Jakubzick 2023 showed that channeling of antigens to CD8+ T cells is facilitated by perforin-2. It translcates the antigens to the cytosol in cross-presenting dendritic cells. A hexadecameric perforin-2 pore forms in phagosome membranes to fåcilitat transport of antigens to the cytosol (Rawat and Jakubzick 2023). Cytosolic antigens use classical MHC-I pathway molecules for cross presentation (ubiquitination, proteasome degradation, and TAP transport into the endoplasmic reticulum).
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| Accession Number: | Q2M385 |
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| Protein Name: | Macrophage-expressed gene 1 protein |
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| Length: | 716 |
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| Molecular Weight: | 78587.00 |
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| Species: | Homo sapiens (Human) [9606] |
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| Number of TMSs: | 1 |
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| Location1 / Topology2 / Orientation3: |
Membrane1 / Single-pass type I membrane protein2 |
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| Substrate |
molecule |
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1: MNNFRATILF WAAAAWAKSG KPSGEMDEVG VQKCKNALKL PVLEVLPGGG WDNLRNVDMG
61: RVMELTYSNC RTTEDGQYII PDEIFTIPQK QSNLEMNSEI LESWANYQSS TSYSINTELS
121: LFSKVNGKFS TEFQRMKTLQ VKDQAITTRV QVRNLVYTVK INPTLELSSG FRKELLDISD
181: RLENNQTRMA TYLAELLVLN YGTHVTTSVD AGAALIQEDH LRASFLQDSQ SSRSAVTASA
241: GLAFQNTVNF KFEENYTSQN VLTKSYLSNR TNSRVQSIGG VPFYPGITLQ AWQQGITNHL
301: VAIDRSGLPL HFFINPNMLP DLPGPLVKKV SKTVETAVKR YYTFNTYPGC TDLNSPNFNF
361: QANTDDGSCE GKMTNFSFGG VYQECTQLSG NRDVLLCQKL EQKNPLTGDF SCPSGYSPVH
421: LLSQIHEEGY NHLECHRKCT LLVFCKTVCE DVFQVAKAEF RAFWCVASSQ VPENSGLLFG
481: GLFSSKSINP MTNAQSCPAG YFPLRLFENL KVCVSQDYEL GSRFAVPFGG FFSCTVGNPL
541: VDPAISRDLG APSLKKCPGG FSQHPALISD GCQVSYCVKS GLFTGGSLPP ARLPPFTRPP
601: LMSQAATNTV IVTNSENARS WIKDSQTHQW RLGEPIELRR AMNVIHGDGG GLSGGAAAGV
661: TVGVTTILAV VITLAIYGTR KFKKKAYQAI EERQSLVPGT AATGDTTYQE QGQSPA