1.C.39.2.8 Perforin 1, PRF1 of 555 aas. Plays a key role in secretory
granule-dependent cell death and in defense against virus-infected and
neoplastic cells. Plays an important role in killing other cells that
are recognized as non-self by the immune system, e.g. in transplant
rejection or some forms of autoimmune disease. Can insert into the
membrane of target cells in its calcium-bound form, oligomerize and form
large pores (Law et al. 2010). Promotes cytolysis and apoptosis of target cells by
facilitating the uptake of cytotoxic granzymes. Perforin gene mutations contribute to hereditary cancer predisposition (Chaudhry et al. 2016). After perforin is secreted by CD8+ cytotoxic T-lymphocytes (CTLs) and disrupts the membranes of extracellular vesicles (EVs), adenosine is released from the EVs and acts as an immunosuppressive metabolite by binding to the adenosine receptor on the CTL membrane. This mechanism provides a novel survival strategy using cancer cell-derived EVs (Tadokoro et al. 2020).
|
Accession Number: | P14222 |
Protein Name: | Perforin-1 |
Length: | 555 |
Molecular Weight: | 61377.00 |
Species: | Homo sapiens (Human) [9606] |
Number of TMSs: | 1 |
Location1 / Topology2 / Orientation3: |
Cytoplasmic granule lumen1 |
Substrate |
molecule |
---|
1: MAARLLLLGI LLLLLPLPVP APCHTAARSE CKRSHKFVPG AWLAGEGVDV TSLRRSGSFP
61: VDTQRFLRPD GTCTLCENAL QEGTLQRLPL ALTNWRAQGS GCQRHVTRAK VSSTEAVARD
121: AARSIRNDWK VGLDVTPKPT SNVHVSVAGS HSQAANFAAQ KTHQDQYSFS TDTVECRFYS
181: FHVVHTPPLH PDFKRALGDL PHHFNASTQP AYLRLISNYG THFIRAVELG GRISALTALR
241: TCELALEGLT DNEVEDCLTV EAQVNIGIHG SISAEAKACE EKKKKHKMTA SFHQTYRERH
301: SEVVGGHHTS INDLLFGIQA GPEQYSAWVN SLPGSPGLVD YTLEPLHVLL DSQDPRREAL
361: RRALSQYLTD RARWRDCSRP CPPGRQKSPR DPCQCVCHGS AVTTQDCCPR QRGLAQLEVT
421: FIQAWGLWGD WFTATDAYVK LFFGGQELRT STVWDNNNPI WSVRLDFGDV LLATGGPLRL
481: QVWDQDSGRD DDLLGTCDQA PKSGSHEVRC NLNHGHLKFR YHARCLPHLG GGTCLDYVPQ
541: MLLGEPPGNR SGAVW