1.C.39.4.7 Gram-negative insecticidal protein, GNIP1Aa of 536 aas and 0 TMSs/ Its structure has been determined to 2.5 Å resolution (PDB# 6FBM) (Zaitseva et al. 2019). It consists of two structurally distinct domains, a MACPF domain and a previously uncharacterized type of domain. GNIP1Aa is unique in being a prokaryotic MACPF member to have both its structure and function identified. It is specifically toxic to Diabrotica virgifera virgifera larvae upon feeding. The MACPF domain is probably important for oligomerization and transmembrane pore formation, while the accompanying domain may define the specificity of the target of toxicity. In GNIP1Aa the accompanying C-terminal domain has a unique fold composed of three pseudosymmetric subdomains with shared sequence similarity, a feature not obvious from the initial sequence examination. This domain is in a family named beta-tripod. Important regions in the beta-tripod domain, which may be involved in target recognition, have been identified (Zaitseva et al. 2019).
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Accession Number: | AML23188.1 |
Protein Name: | AML23188.1 Gram-negative insecticidal protein, partial [Chromobacterium piscinae] |
Length: | 537 |
Molecular Weight: | |
Species: | Chromobacterium piscinae [686831] |
Substrate |
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1: MASAANAGQL GNLPGVTSMG MGYDVNGLYA SPESLLGQPL FDFGGELDSI EIEGRSYTFP
61: RSMHVHTYFH SDFKQDVSKE IEEYREKMSQ HVGVSGRYKL FSASLSVDFT TTDQQLTEIT
121: YSSTREAHVL WYISLPGAAT LRSMLRRDFR DDLNNPNMPA MELFKRYGPY YISEAAVGGR
181: LDYSAASKTL KMDSSQSLST TAEMSYKALV GEIKIEHGSE MEKQVNSFRS NSTIRLTATG
241: GKPGMTDRIL HGPDSQQAFS QWAESLLDYA TLMDFSTESL QPIWALADKP ERRVELEDAF
301: PEFMKQSQQS IPKVDKVLLM DARPPMVKAG EDSGSGASED LAVFNPSTSN GYKMVGQFGQ
361: RNHASVADGH APIFKDLFDL GVLKAPVGWQ RVWDDAGSGK SKDYACWRAI PPQGYRALGD
421: VMMLATSGYN PPNLPDYVCV HQSLCADVQT LQNRVWWDKG TGARKDVSLW QPGAAGAVAS
481: SCFAGVPNYN NPPNSGDIER LRGSIACVKT SAIASMQEMK SMLSQHQGME AMMSKL