TCDB is operated by the Saier Lab Bioinformatics Group
TCIDNameDomainKingdom/PhylumProtein(s)
1.E.36.1.1









Putative holin, Gp33 of 127 aas and 2 TMSs

Viruses
Caudovirales
Gp33 of Mycobacterial phage Cjw1
1.E.36.1.2









Putative holin Gp36 of 84 aas and 2 TMSs (Catalão et al. 2012).

Viruses
Caudovirales
Gp36 of mycobacterial phage PBI1
1.E.36.1.3









Putative holin Gp10 of 137 aas and 2 or 3 TMSs

Viruses
Caudovirales
Gp10 of mycobacterial phage Timshel
1.E.36.1.4









Putative holin, Gp29 of 134 aas and 2 TMSs

Viruses
Caudovirales
Gp29 of mycobacterial phage Charlie
1.E.36.1.5









Putative holin Gp14 of 144 aas and 2 TMSs

Viruses
Caudovirales
Gp14 of mycobacterial phage EricB
1.E.36.1.6









Putative holin of 146 aas and 2 TMSs

Bacteria
Actinobacteria
Putative holin of Mycobacterium massiliense
1.E.36.1.7









Mycobacterial phage D29 holin of 141 aas, Gp11.  Gp11 shows a lipid concentration-dependent conformational switch from an α-helix to a β-sheet structure (Lella and Mahalakshmi 2013). Toxicity has been studied showing that TMS 1 (but not TMS 2) and a C-terminal coiled-coil region are essential for activity because the latter is necessary for holin aggregation, insertion into the membrane and bacterial cell death (Kamilla and Jain 2015).  A role for TMS 2 in pore formation has been proposed (Lella and Mahalakshmi 2016).  The first TMS has been engineered to form a nanopore (Lella and Mahalakshmi 2016).  The D29 and Chy1 phage holins are identical in amino acid sequence (Gan et al. 2016).

Viruses
Caudovirales
Holin, gp11 of Mycobacterial phage D29.
1.E.36.1.8









Putative holin of 138 aas and 2 TMSs

Bacteria
Actinobacteria
Putative holin of Mycobacterium abscessus
1.E.36.1.9









Holin of 136 aas and 2 TMSs, gp255.

Viruses
Caudovirales
gp255 of Mycobacterium phage Pleione
1.E.36.1.10









Uncharacterized protein of 145 aas and 4 TMSs.

Bacteria
Actinobacteria
UP of Demequina flava
1.E.36.2.1









Putative holin of 69 aas and 2 TMSs

Bacteria
Actinobacteria
Putative holin of Actinomyces neuii
1.E.36.2.2









Putative holin of 67 aas and 2 TMSs, Gp31 of mycobacterial phage Brujita

Viruses
Caudovirales
Gp31 of mycobacterial phage Brujita
1.E.36.2.3









Putative holin of 67 aas and 2 TMSs

Viruses
Caudovirales
Gp27 of mycobacterial phage Che9c
1.E.36.2.4









Putative holin of 68 aas and 2 TMSs

Bacteria
Actinobacteria
Putative holin of Mobiluncus mulieris
1.E.36.3.1









Holin of 64 aas and 2 TMSs, HolB (Delisle et al. 2006).

Viruses
Caudovirales
HolB of phage Ar-1
1.E.36.4.1









Putative holin of 178 aas and 2 TMSs, Gp48

Viruses
Caudovirales
Gp48 and mycobacterial phage Ares
1.E.36.5.1









Putative holin of 106 aas and 2 TMSs, Gp54 (Catal√£o et al. 2012).

Viruses
Caudovirales
Gp54 of Mycobacterial phage Omega
1.E.36.5.2









Putative holin of 98 aas and 2 TMSs, Gp71

Viruses
Caudovirales
Gp71 of mycobacterial phage Corndog
1.E.36.6.1









Putative holin, Gp17 of 144 aas and 4 TMSs

Viruses
Caudovirales
Gp17 of Mycobacterial phage Daisy
1.E.36.6.2









Putative holin of 113 aas and 4 TMSs

Bacteria
Actinobacteria
Putative holin of Nocardia farcinica
1.E.36.6.3









Putative holin, Gp16 of 135 aas and 4 TMSs

Viruses
Caudovirales
Gp16 of mycobacterial phage Acadian
1.E.36.6.4









Putative holin, Gp32 of 150 aas and 4 TMSs

Viruses
Caudovirales
Gp32 of Mycobacterial phage Larva
1.E.36.6.5









Putative holin, Gp31 of 128 aas and ~3 TMSs

Viruses
Caudovirales
Gp31 of Mycobacterial phage TM4